Detail of EN22751436


Summary

    Name:LASA_PSEAE
    FullName:Protease lasA,LasA
    Producer Organism:Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
    Sequence Length:418
    Mass:45517
    Calculated Isoelectric Point:9.05
    Target:S. aureus
    Link:P14789
    Function:Involved in proteolysis and elastolysis. Degradation of elastin is likely to contribute to the pathogenicity of P.aeruginosa.

Domains

InterPro

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418

  1  []:IPR011055,Dup_hybrid_motif
  2  []:IPR000841,Pept_M23A_Blytic
  3  []:IPR016047,Peptidase_M23

GO term prediction

  1  GO:0004222, the metalloendopeptidase activity
  2  GO:0006508, the proteolysis

Structures

  •   1
    3IT5    Method:X-ray
    Chains:A/B/E/G=237-418
  •   2
    3IT7    Method:X-ray
    Chains:A/B=237-418

Sequence

MQHKRSRAMASPRSPFLFVLLALAVGGTANAHDDGLPAFRYSAELLGQLQLPSVALPLND
DLFLYGRDAEAFDLEAYLALNAPALRDKSEYLEHWSGYYSINPKVLLTLMVMQSGPLGAP
DERALAAPLGRLSAKRGFDAQVRDVLQQLSRRYYGFEEYQLRQAAARKAVGEDGLNAASA
ALLGLLREGAKVSAVQGGNPLGAYAQTFQRLFGTPAAELLQPSNRVARQLQAKAALAPPS
NLMQLPWRQGYSWQPNGAHSNTGSGYPYSSFDASYDWPRWGSATYSVVAAHAGTVRVLSR
CQVRVTHPSGWATNYYHMDQIQVSNGQQVSADTKLGVYAGNINTALCEGGSSTGPHLHFS
LLYNGAFVSLQGASFGPYRINVGTSNYDNDCRRYYFYNQSAGTTHCAFRPLYNPGLAL
  BlastP to EnzyBase2

Activity

    No MICs found on enzybiotics database

References

  [1]  Warrener P.,Mizoguchi S.D.,Erwin A.L.,Pham X.-Q.T.,Stover C.K.,  Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen.  Nature,2000,(406):959-964  [MEDLINE:20437337]
  [2]  Ohman D.E.,Kessler E.,Gustin J.K.,  A substitution at His-120 in the LasA protease of Pseudomonas aeruginosa blocks enzymatic activity without affecting propeptide processing or extracellular secretion.  J. Bacteriol.,1996,(178):6608-6617  [MEDLINE:97086629]
  [3]  Ohman D.E.,Olson J.C.,  Efficient production and processing of elastase and LasA by Pseudomonas aeruginosa require zinc and calcium ions.  J. Bacteriol.,1992,(174):4140-4147  [MEDLINE:92283772]
  [4]  Galloway D.R.,Peters J.E.,Darzins A.,  Revised nucleotide sequence of the lasA gene from Pseudomonas aeruginosa PAO1.  Nucleic Acids Res.,1990,(18):6444-6444  [MEDLINE:91057149]
  [5]  Iglewski B.H.,Schad P.A.,  Nucleotide sequence and expression in Escherichia coli of the Pseudomonas aeruginosa lasA gene.  J. Bacteriol.,1988,(170):2784-2789  [MEDLINE:88227864]