Detail of EN24607609


Summary

    Name:LYTM_STAA8
    FullName:Glycyl-glycine endopeptidase lytM,LytM
    Producer Organism:Staphylococcus aureus (strain NCTC 8325)
    Sequence Length:316
    Mass:34317
    Calculated Isoelectric Point:6.68
    Target:S. aureus,S. carnosus
    Link:O33599
    Function:Peptidoglycan hydrolase (autolysin) specifically acting on polyglycine interpeptide bridges of the cell wall peptidoglycan.

Domains

InterPro

50
100
150
200
250
300
316

  1  []:IPR011055,Dup_hybrid_motif
  2  []:IPR016047,Peptidase_M23

GO term prediction

    No GO term prediction

Structures

  •   1
    1QWY    Method:X-ray
    Chains:A=26-316
  •   2
    2B0P    Method:X-ray
    Chains:A/B=185-316
  •   3
    2B13    Method:X-ray
    Chains:A/B=185-316
  •   4
    2B44    Method:X-ray
    Chains:A/B=185-316

Sequence

MKKLTAAAIATMGFATFTMAHQADAAETTNTQQAHTQMSTQSQDVSYGTYYTIDSNGDYH
HTPDGNWNQAMFDNKEYSYTFVDAQGHTHYFYNCYPKNANANGSGQTYVNPATAGDNNDY
TASQSQQHINQYGYQSNVGPDASYYSHSNNNQAYNSHDGNGKVNYPNGTSNQNGGSASKA
TASGHAKDASWLTSRKQLQPYGQYHGGGAHYGVDYAMPENSPVYSLTDGTVVQAGWSNYG
GGNQVTIKEANSNNYQWYMHNNRLTVSAGDKVKAGDQIAYSGSTGNSTAPHVHFQRMSGG
IGNQYAVDPTSYLQSR
  BlastP to EnzyBase2

Activity

    No MICs found on enzybiotics database

References

  [1]  Tsompanidou E.,Buist G.,van der Kooi-Pol M.M.,Winter T.,Sibbald M.J.,  Synthetic effects of secG and secY2 mutations on exoproteome biogenesis in Staphylococcus aureus.  J. Bacteriol.,2010,(192):3788-3800  [PubMed:20472795]
  [2]  Bochtler M.,Marcyjaniak M.,Sabala I.,Odintsov S.G.,  Latent LytM at 1.3A resolution.  J. Mol. Biol.,2004,(335):775-785  [PubMed:14687573]
  [3]  Cheung A.L.,Van Wamel W.,Ingavale S.S.,  Characterization of RAT, an autolysis regulator in Staphylococcus aureus.  Mol. Microbiol.,2003,(48):1451-1466  [MEDLINE:22676862]
  [4]  Jayaswal R.K.,Nadakavukaren M.J.,Lockwood K.J.,Ramadurai L.,  Characterization of a chromosomally encoded glycylglycine endopeptidase of Staphylococcus aureus.  Microbiology,1999,(145):801-808  [MEDLINE:99235565]
  [5]  Jayaswal R.K.,Ramadurai L.,  Molecular cloning, sequencing, and expression of lytM, a unique autolytic gene of Staphylococcus aureus.  J. Bacteriol.,1997,(179):3625-3631  [MEDLINE:97315235]
  [6]  Jayaswal R.K.,Tobin P.,Mani N.,  Isolation and characterization of autolysis-defective mutants of Staphylococcus aureus created by Tn917-lacZ mutagenesis.  J. Bacteriol.,1993,(175):1493-1499  [MEDLINE:93186718]