Detail of EN66985644

Summary

    Name:ALYS_STRPN
    FullName:Autolysin,LytA
    Producer Organism:Streptococcus pneumoniae
    Sequence Length:318
    Mass:36544
    Calculated Isoelectric Point:4.99
    Target:S. pneumoniae
    Link:P06653
    Function:Autolysins are involved in some important biological processes such as cell separation, cell-wall turnover, competence for genetic transformation, formation of the flagella and sporulation. Autolysin strictly depends on the presence of choline-containing cell walls for activity.

Domains

IPR002502:10 - 170

IPR018337:175 - 194

IPR018337:196 - 215

IPR018337:217 - 237

IPR018337:238 - 257

IPR018337:258 - 277

IPR018337:280 - 301

InterPro

100

150

200

250

300

318

  1  [10 - 170]:IPR002502,N-acetylmuramoyl-L-alanine amidase domain
  2  [175 - 194]:IPR018337,Cell wall/choline-binding repeat
  3  [196 - 215]:IPR018337,Cell wall/choline-binding repeat
  4  [217 - 237]:IPR018337,Cell wall/choline-binding repeat
  5  [238 - 257]:IPR018337,Cell wall/choline-binding repeat
  6  [258 - 277]:IPR018337,Cell wall/choline-binding repeat
  7  [280 - 301]:IPR018337,Cell wall/choline-binding repeat

GO term prediction

1 GO:0008745, the N-acetylmuramoyl-L-alanine amidase activity
2 GO:0009253, the peptidoglycan catabolic process

Structures

1
1GVMMethod:X-ray
Chains:A/B/C/D/E/F=188-318
2
1H8GMethod:X-ray
Chains:A/B=224-318
3
1HCXMethod:X-ray
Chains:A/B=192-318
4
2BMLMethod:X-ray
Chains:A/B=193-318

Sequence

MEINVSKLRTDLPQVGVQPYRQVHAHSTGNPHSTVQNEADYHWRKDPELGFFSHIVGNGC
IMQVGPVDNGAWDVGGGWNAETYAAVELIESHSTKEEFMTDYRLYIELLRNLADEAGLPK
TLDTGSLAGIKTHEYCTNNQPNNHSDHVDPYPYLAKWGISREQFKHDIENGLTIETGWQK
NDTGYWYVHSDGSYPKDKFEKINGTWYYFDSSGYMLADRWRKHTDGNWYWFDNSGEMATG
WKKIADKWYYFNEEGAMKTGWVKYKDTWYYLDAKEGAMVSNAFIQSADGTGWYYLKPDGT
LADKPEFTVEPDGLITVK

BlastP to EnzyBase2

Activity

No MICs found on enzybiotics database

References

[1] Read T.D.,Eisen J.A.,Paulsen I.T.,Nelson K.E.,Tettelin H., Complete genome sequence of a virulent isolate of Streptococcus pneumoniae. Science,2001,(293):498-506 [MEDLINE:21357209]
[2] Lopez R.,Garcia E.,Garcia J.L.,Garcia P., Nucleotide sequence and expression of the pneumococcal autolysin gene from its own promoter in Escherichia coli. Gene,1986,(43):265-272 [MEDLINE:86301881]