Detail of EN6984837

Summary

    Name:MEPA_ECOLI
    FullName:Penicillin-insensitive murein endopeptidase,MepA
    Producer Organism:Escherichia coli (strain K12)
    Sequence Length:274
    Mass:30137
    Calculated Isoelectric Point:8.4
    Target:E. coli
    Link:P0C0T5
    Function:Involved in the removal of murein from the sacculus. May also facilitate integration of nascent murein strands into the sacculus by cleaving the peptide bonds between neighboring strands in mature murein.

Domains

InterPro

100

150

200

250

274

1 []:IPR009045,Hedgehog/DD-peptidase
2 []:IPR005073,Peptidase_M74

GO term prediction

  1  GO:0004252, the serine-type endopeptidase activity
  2  GO:0006508, the proteolysis
  3  GO:0030288, the outer membrane-bounded periplasmic space

Structures

1
1TZPMethod:X-ray
Chains:A/B=20-274
2
1U10Method:X-ray
Chains:A/B/C/D/E/F=20-274

Sequence

MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGCIVGADTLPIQSEHYQV
MRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPAGGRFNGGHASHQTGLDVD
IFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEIFSLIKLAAQDKDVTRIFVNP
AIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPADSLECEDQPLPPSGDGCGAELQ
SWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI

BlastP to EnzyBase2

Activity

No MICs found on enzybiotics database

References

  [1]  Isono K.,Fujita K.,Yamamoto Y.,Morooka N.,Hayashi K.,  Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.  Mol. Syst. Biol.,2006,(2):0-0  [PubMed:16738553]
  [2]  Bochtler M.,Sabala I.,Odintsov S.G.,Marcyjaniak M.,  Peptidoglycan amidase MepA is a LAS metallopeptidase.  J. Biol. Chem.,2004,(279):43982-43989  [PubMed:15292190]
  [3]  Inada T.,Hayashi K.,Baba T.,Aiba H.,Yamamoto Y.,  Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features.  DNA Res.,1997,(4):91-113  [MEDLINE:97349980]
  [4]  Burland V.,Perna N.T.,Bloch C.A.,Plunkett G. III,Blattner F.R.,  The complete genome sequence of Escherichia coli K-12.  Science,1997,(277):1453-1474  [MEDLINE:97426617]
  [5]  Goodell E.W.,Huber M.,van Leeuwen A.M.,Keck W.,  Cloning and characterization of mepA, the structural gene of the penicillin-insensitive murein endopeptidase from Escherichia coli.  Mol. Microbiol.,1990,(4):209-219  [MEDLINE:90251161]