Detail of EN77716314

Summary

    Name:ALYS_BPHB3
    FullName:phage HB-3 lysin,HBL
    Producer Organism:Streptococcus pneumoniae phage HB-3
    Sequence Length:318
    Mass:36509
    Calculated Isoelectric Point:5.09
    Target:S. pneumoniae
    Link:P32762
    Function:Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides.

Domains

IPR002502:10-170

IPR018337:175-194

IPR018337:196-215

IPR018337:217-237

IPR018337:238-257

IPR018337:258-277

IPR018337:280-301

InterPro

100

150

200

250

300

318

  1  [10-170]:IPR002502,N-acetylmuramoyl-L-alanine amidase domain
  2  [175-194]:IPR018337,Cell wall/choline-binding repeat?
  3  [196-215]:IPR018337,Cell wall/choline-binding repeat?
  4  [217-237]:IPR018337,Cell wall/choline-binding repeat?
  5  [238-257]:IPR018337,Cell wall/choline-binding repeat?
  6  [258-277]:IPR018337,Cell wall/choline-binding repeat?
  7  [280-301]:IPR018337,Cell wall/choline-binding repeat?

GO term prediction

1 GO:0008745, the N-acetylmuramoyl-L-alanine amidase activity
2 GO:0009253, the peptidoglycan catabolic process

Structures

No structs found on enzybiotics database

Sequence

MDIDRNRLRTGLPQVGVQPYRQVHAHSTGNRNSTVQNEADYHWRKDPELGFFSHVVGNFR
IMQVGPVNNGSWDVGGGWNAETYAAVELIESHSTKEEFMADYRLYIELLRNLADEAGLPK
TLDTDDLAGIKTHEYCTNNQPNNHSDHVDPYPYLASWGISREQFKQDIENGLSAATGWQK
NGTGYWYVHSDGSYSKDKFEKINGTWYYFDGSGYMLSDRWKKHTDGNWYYFDQSGEMATG
WKKIADKWYYFDVEGAMKTGWVKYKDTWYYLDAKEGAMVSNAFIQSADGTGWYYLKPDGT
LADKPEFTVEPDGLITVK

BlastP to EnzyBase2

Activity

No MICs found on enzybiotics database

References

[1] Garcia P.,Lopez R.,Romero A., Sequence of the Streptococcus pneumoniae bacteriophage HB-3 amidase reveals high homology with the major host autolysin. J. Bacteriol.,1990,(172):5064-5070 [MEDLINE:90368559]
[2] Garcia P.,Lopez R.,Romero A., Characterization of the pneumococcal bacteriophage HB-3 amidase: cloning and expression in Escherichia coli. J. Virol.,1990,(64):137-142 [MEDLINE:90080114]