Detail of EN9623861

Summary

    Name:CWLK_BACSU
    FullName:Peptidoglycan L-alanyl-D-glutamate endopeptidase CwlK
    Producer Organism:Bacillus subtilis
    Sequence Length:167
    Mass:19113
    Calculated Isoelectric Point:6.01
    Target:B. megaterium
    Link:O34360
    Function:Cleaves the linkage of the L-alanine-D-glutamic acid of B.subtilis cell wall.

Domains

InterPro

100

150

167

  1  []:IPR009045,Hedgehog/DD-peptidase
  2  []:IPR012340,NA-bd_OB-fold
  3  []:IPR003709,Pept_M15B/M15C

GO term prediction

1 GO:0008233, the peptidase activity
2 GO:0006508, the proteolysis

Structures

No structs found on enzybiotics database

Sequence

MNLPAKTFVILCILFLLDLCFSYIRHEWHSQNALQDMPVPSDLHPIVKQNADALKAAAAN
KGIDVVITEGFRSFKEQDELYKQGRTKKGNIVTYARGGESYHNYGLAIDFALQKKDGSII
WDMEYDGNQNGKSDWLEVVEIAKTLGFEWGGDWKRFKDYPHLEMIPN

BlastP to EnzyBase2

Activity

No MICs found on enzybiotics database

References

  [1]  Sekiguchi J.,Yamamoto H.,Kitajima T.,Yao Y.,Fukushima T.,  Characterization of new L,D-endopeptidase gene product CwlK (previous YcdD) that hydrolyzes peptidoglycan in Bacillus subtilis.  Mol. Genet. Genomics,2007,(278):371-383  [PubMed:17588176]
  [2]  Yamane K.,Tamakoshi A.,Kumano M.,  A 32 kb nucleotide sequence from the region of the lincomycin-resistance gene (22 degrees-25 degrees) of the Bacillus subtilis chromosome and identification of the site of the lin-2 mutation.  Microbiology,1997,(143):2775-2782  [MEDLINE:97419516]
  [3]  Alloni G.,Albertini A.M.,Moszer I.,Ogasawara N.,Kunst F.,  The complete genome sequence of the Gram-positive bacterium Bacillus subtilis.  Nature,1997,(390):249-256  [MEDLINE:98044033]