Detail of GM0005


Summary

The genetically modified enzybiotic, named CHL , constructed by Domains Assembly strategy, change or extend lytic spectrum.
With  CHL
  Sequence Length:  501 AA.
  Mass:  57305 Da.
  Isoelectric Point:  4.57
  Function:  The chimeric enzyme behaves as a chuline-dependent enzyme and its activity is comparable to that of the parent enzymes.

Construction

GM0005, constructed by Domains Assembly strategy.
P15057: CP-1 lysin
P32762: Lytic amidase
UniProt

IPR017853:3-185
IPR018337:220-239
IPR018337:241-260
IPR018337:261-280
IPR018337:281-301
IPR018337:303-322
IPR002502:10-170
IPR018337:175-194
IPR018337:196-215
IPR018337:217-237
IPR018337:238-257
IPR018337:258-277
IPR018337:280-301
InterPro

GM0005:1-185
GM0005:186-503
GMEnzy

30
60
90
120
150
180
210
240
270
300
330
339
30
60
90
120
150
180
210
240
270
300
318

Details:
GM0005[1-185]: derive from protein P15057 sequence 1-185

P15057, 339 AA., the CP-1 lysin from Streptococcus phage Cp-1

Source: Streptococcus phage Cp-1

Domains and repeats

3-185:  IPR017853, the Glycoside hydrolase, catalytic domain?
220-239:  IPR018337, the Cell wall/choline-binding repeat
241-260:  IPR018337, the Cell wall/choline-binding repeat
261-280:  IPR018337, the Cell wall/choline-binding repeat
281-301:  IPR018337, the Cell wall/choline-binding repeat
303-322:  IPR018337, the Cell wall/choline-binding repeat

GO term prediction

Biological Process:  0005975, the carbohydrate metabolic process
Biological Process:  0009253, the peptidoglycan catabolic process
Biological Process:  0016998, the cell wall macromolecule catabolic process
Molecular Function:  0003796, the lysozyme activity


Linking to UniprotKB
Linging to InterPro
GM0005[186-503]: derive from protein P32762 sequence 1-318

P32762, 318 AA., the Lytic amidase from Streptococcus pneumoniae phage HB-3

Source: Streptococcus pneumoniae phage HB-3

Domains and repeats

10-170:  IPR002502, the N-acetylmuramoyl-L-alanine amidase domain
175-194:  IPR018337, the Cell wall/choline-binding repeat?
196-215:  IPR018337, the Cell wall/choline-binding repeat?
217-237:  IPR018337, the Cell wall/choline-binding repeat?
238-257:  IPR018337, the Cell wall/choline-binding repeat?
258-277:  IPR018337, the Cell wall/choline-binding repeat?
280-301:  IPR018337, the Cell wall/choline-binding repeat?

GO term prediction

Molecular Function:  0009253, the peptidoglycan catabolic process
Molecular Function:  0008745, the N-acetylmuramoyl-L-alanine amidase activity


Linking to UniprotKB
Linging to InterPro

Annotation

1-185
186-503
50
100
150
200
250
300
350
400
450
500
550
600
650
657

1-183
: EAD, Lysozyme domain, derived from Pneumococcal phage Cp-1 endolysin Cpl1;
184-318
: EAD, Amidase domain, derived from Pneumococcal phage endolysin HBL3;
319-482
: CBD, choline-binding domain, derived from Pneumococcal phage endolysin HBL3;
Domain and repeats:
195-355
: IPR002502,N-acetylmuramoyl-L-alanine amidase domain;
3-185
: IPR017853,Glycoside hydrolase, catalytic domain?;
360-379
: IPR018337,Cell wall/choline-binding repeat?;
381-400
: IPR018337,Cell wall/choline-binding repeat?;
402-422
: IPR018337,Cell wall/choline-binding repeat?;
423-442
: IPR018337,Cell wall/choline-binding repeat?;
443-462
: IPR018337,Cell wall/choline-binding repeat?;
465-486
: IPR018337,Cell wall/choline-binding repeat?;
GO term prediction:
0008745, the N-acetylmuramoyl-L-alanine amidase activity
0009253, the peptidoglycan catabolic process
DLFVDVSSHNGYDITGILEQMGTTNTIIKISESTTYLNPCLSAQVEQSNPIGFYHFARFGGDVAEAEREAQFFLDNVPMQVKYLVLDYEDDPSGDAQANTNACLRFMQMIADAGYKPIYYSYKPFTHDNVDYQQILAQFPNSLWIAGYGLNDGTANFEYFPSMDGIRWWQYSSNPFDKNIVLL-MDIDRNRLRTGLPQVGVQPYRQVHAHSTGNRNSTVQNEADYHWRKDPELGFFSHVVGNFRIMQVGPVNNGSWDVGGGWNAETYAAVELIESHSTKEEFMADYRLYIELLRNLADEAGLPKTLDTDDLAGIKTHEYCTNNQPNNHSDHVDPYPY-LASWGISREQFKQDIENGLSAATGWQKNGTGYWYVHSDGSYSKDKFEKINGTWYYFDGSGYMLSDRWKKHTDGNWYYFDQSGEMATGWKKIADKWYYFDVEGAMKTGWVKYKDTWYYLDAKEGAMVSNAFIQSADGTGWYYLKPDGTLADKPEFTVEPDGLITVK
  BlastP to GMEnzy

Production

  1.  Expressed by in E. coli TG1,  Purified by affinity chromatography on DEAE-cellulose

Activity

    No activity data found on GM0005

Reference

  1. Jesus M. SANZ, Pedro GARCIA and Josei. CARCIA. (1996) Construction of a multifunctional pneumococcal murein hydrolase by module assembly. Eur. J. Biochem.. 235:601-605. [doi:10.1111/j.1432-1033.1996.00601.x] [PMID:8654407]

Comments

    No comments found on GM0005


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