Detail of GM0006


Summary

The genetically modified enzybiotic, named LYZ161 , constructed by Truncation strategy, undisclosed.
With  LYZ161
  Sequence Length:  161 AA.
  Mass:  17961.3 Da.
  Isoelectric Point:  10.27
  Function:  could not be detected after nickel column purification of the E. coli

Construction

GM0006, constructed by Truncation strategy.
B7T0E8: Gp58
UniProt

IPR007921:15-149
IPR013338:483-629
InterPro

GM0006:1-161
GMEnzy

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Details:
GM0006[1-161]: derive from protein B7T0E8 sequence 475-634

B7T0E8, 634 AA., the Gp58 from Staphylococcus phage phiSauS-IPLA88

Source: Staphylococcus phage phiSauS-IPLA88

Domains and repeats

15-149:  IPR007921, the CHAP domain
483-629:  IPR013338, the Lysozyme domain, subfamily 2

GO term prediction

Biological Process:  0009253, the peptidoglycan catabolic process
Biological Process:  0044036, the cell wall macromolecule metabolic process
Molecular Function:  0004040, the amidase activity?
Molecular Function:  0016787, the hydrolase activity?


Linking to UniprotKB
Linging to InterPro

Annotation

1-161
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634

1-161
: EAD, lysozyme subfamily 2 lytic domain domain, derived from Staphylococcus aureus bacteriophage vB_SauS-phi-IPLA88 endolysin HydH5;
Domain and repeats:
9-156
: IPR013338,Lysozyme domain, subfamily 2;
GO term prediction:
0016787, the hydrolase activity
GVSVSSLNKILKGKGTLNNQGKAFAEACKKHNINEIYLIAHAFLESGYGTSNFASGKDGVYNYFGIGAYDNNPNYAMTFARNKGWTSPAKAIMGGASFVRKDYINKGQNTLYRIRWNPKNPATHQYATAIEWCQHQASTIAKLYKQIGLKGVYFTRDKYKL
  BlastP to GMEnzy

Production

  1.  Expressed by pET21a in E. coli BL21(DE3),  Purified by NiNTA nickel column chromatography (Qiagen, Valencia, CA).

Activity

    No activity data found on GM0006

Reference

  1. Lorena Rodriguez-Rubio, Beatriz Martinez, Ana Rodriguez1, David M. Donovan, and Pilar Garc¨ªa. (2012) Enhanced staphylolytic activity of the Staphylococcus aureus bacteriophage vB_SauS-phiIPLA88 HydH5 virion associated peptidoglycan hydrolase: fusions, deletions and synergy with LysH5. Appl. Environ. Microbiol.. 4431-2:32-41. [doi:10.1128/AEM.07621-11] [PMID:22267667] [FULL TEXT]

Comments

    No comments found on GM0006


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