Detail of GM0016
Summary
The genetically modified enzybiotic, named LysMAcmA-PMBMTH719 fusion protein , constructed by Domains Assembly strategy, change or extend lytic spectrum.With LysMAcmA-PMBMTH719 fusion protein
Sequence Length: 228 AA.
Mass: 25652.6 Da.
Isoelectric Point: 10.98
Function: The M–P fusion protein is capable of binding to murein,pseudomurein, and chitin
Construction
GM0016, constructed by Domains Assembly strategy. No schema construted on GM0016
Details:
GM0016[1-129]: derive from protein P0C2T5 sequence 244-437
P0C2T5, 437 AA., the Probable N-acetylmuramidase from Lactococcus lactis subsp. cremoris (Streptococcus
Source: Lactococcus lactis subsp. cremoris (Streptococcus
244-287: IPR018392, the LysM domain?
320-362: IPR018392, the LysM domain?
394-437: IPR018392, the LysM domain?
Biological Process: 0044036, the cell wall macromolecule metabolic process
Biological Process: 0016998, the cell wall macromolecule catabolic process?
Molecular Function: 0004040, the amidase activity?
Molecular Function: 0016787, the hydrolase activity?
Linking to UniprotKB
Linging to InterPro
Domains and repeats
57-214: IPR013338, the Lysozyme domain, subfamily 2244-287: IPR018392, the LysM domain?
320-362: IPR018392, the LysM domain?
394-437: IPR018392, the LysM domain?
GO term prediction
Biological Process: 0009253, the peptidoglycan catabolic processBiological Process: 0044036, the cell wall macromolecule metabolic process
Biological Process: 0016998, the cell wall macromolecule catabolic process?
Molecular Function: 0004040, the amidase activity?
Molecular Function: 0016787, the hydrolase activity?
Linking to UniprotKB
Linging to InterPro
GM0016[130-228]: derive from protein O26815 sequence 433-574
O26815, 574 AA., the Cell surface glycoprotein (S-layer protein) from Methanothermobacter thermautotrophicus (strain ATC
Source: Methanothermobacter thermautotrophicus (strain ATC
255-371: IPR012334, the Pectin lyase fold
111-153: IPR022441, the Parallel beta-helix repeat-2
155-198: IPR022441, the Parallel beta-helix repeat-2
433-464: IPR018975, the Pseudomurein-binding repeat
510-538: IPR018975, the Pseudomurein-binding repeat
543-574: IPR018975, the Pseudomurein-binding repeat
Linking to UniprotKB
Linging to InterPro
Domains and repeats
19-239: IPR012334, the Pectin lyase fold255-371: IPR012334, the Pectin lyase fold
111-153: IPR022441, the Parallel beta-helix repeat-2
155-198: IPR022441, the Parallel beta-helix repeat-2
433-464: IPR018975, the Pseudomurein-binding repeat
510-538: IPR018975, the Pseudomurein-binding repeat
543-574: IPR018975, the Pseudomurein-binding repeat
GO term prediction
No GO info found on GM0016Linking to UniprotKB
Linging to InterPro
Annotation
1-129
130-228
1-129
: CBD, Cell wall-targeting domain, derived from autolysin??AcmA?? from Lactococcus lactis;130-228
: CBD, pseudomurein cell wall-binding domain, derived from S-layer protein MTH719 from Methanothermobacter thermautotrophicus;Domain and repeats:
1--21
: IPR018392,LysM domain?; 130-118
: IPR018975,Pseudomurein-binding repeat; 151-129
: IPR018392,LysM domain?; 207-192
: IPR018975,Pseudomurein-binding repeat; 240-228
: IPR018975,Pseudomurein-binding repeat; 77-54
: IPR018392,LysM domain?; GO term prediction:
YTVKSGDTLWGISQRYGISVAQIQSANNLKSTIIYIGQKLVLT-VKVKSGDTLWALSVKYKTSIAQLKSWNHLSSDTIYIGQNLIVS-HKVVKGDTLWGLSQKSGSPIASIKAWNHLSSDTILIGQYLRIK-ASVKISVRQLTDAASWVRAYYGRYGRLPGKVTI-RHGRLYRSSYVRVAKNILSFIDSRGRAPNYAIT-GLGRIPFQRLVYMYSKIIKFYGTNKRLPNYVTI