Detail of GM0016


Summary

The genetically modified enzybiotic, named LysMAcmA-PMBMTH719 fusion protein , constructed by Domains Assembly strategy, change or extend lytic spectrum.
With  LysMAcmA-PMBMTH719 fusion protein
  Sequence Length:  228 AA.
  Mass:  25652.6 Da.
  Isoelectric Point:  10.98
  Function:  The M–P fusion protein is capable of binding to murein,pseudomurein, and chitin

Construction

GM0016, constructed by Domains Assembly strategy.
    No schema construted on GM0016
Details:
GM0016[1-129]: derive from protein P0C2T5 sequence 244-437

P0C2T5, 437 AA., the Probable N-acetylmuramidase from Lactococcus lactis subsp. cremoris (Streptococcus

Source: Lactococcus lactis subsp. cremoris (Streptococcus

Domains and repeats

57-214:  IPR013338, the Lysozyme domain, subfamily 2
244-287:  IPR018392, the LysM domain?
320-362:  IPR018392, the LysM domain?
394-437:  IPR018392, the LysM domain?

GO term prediction

Biological Process:  0009253, the peptidoglycan catabolic process
Biological Process:  0044036, the cell wall macromolecule metabolic process
Biological Process:  0016998, the cell wall macromolecule catabolic process?
Molecular Function:  0004040, the amidase activity?
Molecular Function:  0016787, the hydrolase activity?


Linking to UniprotKB
Linging to InterPro
GM0016[130-228]: derive from protein O26815 sequence 433-574

O26815, 574 AA., the Cell surface glycoprotein (S-layer protein) from Methanothermobacter thermautotrophicus (strain ATC

Source: Methanothermobacter thermautotrophicus (strain ATC

Domains and repeats

19-239:  IPR012334, the Pectin lyase fold
255-371:  IPR012334, the Pectin lyase fold
111-153:  IPR022441, the Parallel beta-helix repeat-2
155-198:  IPR022441, the Parallel beta-helix repeat-2
433-464:  IPR018975, the Pseudomurein-binding repeat
510-538:  IPR018975, the Pseudomurein-binding repeat
543-574:  IPR018975, the Pseudomurein-binding repeat

GO term prediction

    No GO info found on GM0016

Linking to UniprotKB
Linging to InterPro

Annotation

1-129
130-228

1-129
: CBD, Cell wall-targeting domain, derived from autolysin??AcmA?? from Lactococcus lactis;
130-228
: CBD, pseudomurein cell wall-binding domain, derived from S-layer protein MTH719 from Methanothermobacter thermautotrophicus;
Domain and repeats:
1--21
: IPR018392,LysM domain?;
130-118
: IPR018975,Pseudomurein-binding repeat;
151-129
: IPR018392,LysM domain?;
207-192
: IPR018975,Pseudomurein-binding repeat;
240-228
: IPR018975,Pseudomurein-binding repeat;
77-54
: IPR018392,LysM domain?;
GO term prediction:
YTVKSGDTLWGISQRYGISVAQIQSANNLKSTIIYIGQKLVLT-VKVKSGDTLWALSVKYKTSIAQLKSWNHLSSDTIYIGQNLIVS-HKVVKGDTLWGLSQKSGSPIASIKAWNHLSSDTILIGQYLRIK-ASVKISVRQLTDAASWVRAYYGRYGRLPGKVTI-RHGRLYRSSYVRVAKNILSFIDSRGRAPNYAIT-GLGRIPFQRLVYMYSKIIKFYGTNKRLPNYVTI
  BlastP to GMEnzy

Production

  1.  Expressed by in E. coli Rosetta gami 2,  Purified by NiNTA nickel column chromatography ((Ni-NTA superflow,QiagenGmbH,Hilden, Germany).

Activity

    No activity data found on GM0016

Reference

  1. Visweswaran, G. R.Dijkstra, B. W.Kok, J.. (2012) A genetically engineered protein domain binding to bacterial murein, archaeal pseudomurein, and fungal chitin cell wall material. Applied microbiology and biotechnology. 03:729-737. [doi:10.1007/s00253-012-3871-0] [PMID:22262228] [FULL TEXT]

Comments

    No comments found on GM0016


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