Detail of GM0019


Summary

The genetically modified enzybiotic, named Lys168-87 , constructed by Domains Assembly strategy, enhance the solubility,change or extend lytic spectrum.
With  Lys168-87
  Sequence Length:  264 AA.
  Mass:  29159.9 Da.
  Isoelectric Point:  9.11
  Function:  very effective in lysing S. aureus clinical isolates,Lys168-87 again showed a better lytic performance.

Construction

GM0019, constructed by Domains Assembly strategy.
S5MRN1: N-acetylmuramoyl-L-alanine amidase
U5T1I9: Phage lysin, N-acetylmuramoyl-L-alanine amidase
UniProt

IPR007921:4-137
IPR007921:4-133
InterPro

GM0019:1-150
GM0019:185-264
GMEnzy

30
60
90
120
150
180
210
234
30
60
90
120
150
180
210
240
251

Details:
GM0019[1-150]: derive from protein S5MRN1 sequence 1-150

S5MRN1, 234 AA., the N-acetylmuramoyl-L-alanine amidase from Bacillus anthracis str. A16R

Source: Bacillus anthracis str. A16R

Domains and repeats

4-137:  IPR007921, the CHAP domain

GO term prediction

    No GO info found on GM0019

Linking to UniprotKB
Linging to InterPro
GM0019[185-264]: derive from protein U5T1I9 sequence 151-255

U5T1I9, 251 AA., the Phage lysin, N-acetylmuramoyl-L-alanine amidase from Staphylococcus aureus subsp. aureus Z172

Source: Staphylococcus aureus subsp. aureus Z172

Domains and repeats

4-133:  IPR007921, the CHAP domain

GO term prediction

    No GO info found on GM0019

Linking to UniprotKB
Linging to InterPro

Annotation

1-150
185-264
50
100
150
200
250
300
350
400
450
485

1-137
: EAD, CHAP domain, derived from Enterococcus faecalis phage F170/08 endolysins Lys168;
185-264
: CBD, Cell wall-targeting domain, derived from staphylococcal phage endolysin Lys87;
Domain and repeats:
4-137
: IPR007921,CHAP domain;
GO term prediction:
MVKLNDVLSYVNGLVGKGVDADGWYGTQCMDLTVDVMQRFFGWRPYGNAIALVDQPLPAGFQRIRTTSSTQIKAGDVMIWGLGYYAQYGHTGIATEDGRADGTFVSVDQNWINPSLEVGSPAAAIHHNMDGVWGVIRPPYEAASTPKPPAPKPDKPNLGQ-KVKPPAQKAVGKSASKITVGSKAPYNLKWSKGAYFNAKIDGLGATSATRYGDNRTNYRFDVGQAVYAPGTLIYVFEIIDGWCRIYWNNHNEWIWHERLIVKEVF
  BlastP to GMEnzy

Production

  1.  Expressed by pIVEX2.3d in E. coli XL1-Blue,  Purified by affinity chromatography using HisTrapTM HP columns (GE Healthcare) coupled to an KTA-Prime system(GEHealthcare).

Activity

    No activity data found on GM0019

Reference

  1. Fernandes, S.Proenca, D.Cantante, C.Silva, F. A.Leandro, C.Lourenco, S.Milheirico, C.de Lencastre, H. (2012) Novel chimerical endolysins with broad antimicrobial activity against methicillin-resistant Staphylococcus aureus. . 03:333-343. [doi:10.1089/mdr.2012.0025] [PMID:22432707]

Comments

    No comments found on GM0019


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