Detail of GM0019
Summary
The genetically modified enzybiotic, named Lys168-87 , constructed by Domains Assembly strategy, enhance the solubility,change or extend lytic spectrum.With Lys168-87
Sequence Length: 264 AA.
Mass: 29159.9 Da.
Isoelectric Point: 9.11
Function: very effective in lysing S. aureus clinical isolates,Lys168-87 again showed a better lytic performance.
Construction
GM0019, constructed by Domains Assembly strategy. No schema construted on GM0019
Details:
GM0019[1-150]: derive from protein S5MRN1 sequence 1-150
S5MRN1, 234 AA., the N-acetylmuramoyl-L-alanine amidase from Bacillus anthracis str. A16R
Source: Bacillus anthracis str. A16R
Linking to UniprotKB
Linging to InterPro
Domains and repeats
4-137: IPR007921, the CHAP domainGO term prediction
No GO info found on GM0019Linking to UniprotKB
Linging to InterPro
GM0019[185-264]: derive from protein U5T1I9 sequence 151-255
U5T1I9, 251 AA., the Phage lysin, N-acetylmuramoyl-L-alanine amidase from Staphylococcus aureus subsp. aureus Z172
Source: Staphylococcus aureus subsp. aureus Z172
Linking to UniprotKB
Linging to InterPro
Domains and repeats
4-133: IPR007921, the CHAP domainGO term prediction
No GO info found on GM0019Linking to UniprotKB
Linging to InterPro
Annotation
1-150
185-264
1-137
: EAD, CHAP domain, derived from Enterococcus faecalis phage F170/08 endolysins Lys168;185-264
: CBD, Cell wall-targeting domain, derived from staphylococcal phage endolysin Lys87;Domain and repeats:
4-137
: IPR007921,CHAP domain; GO term prediction:
MVKLNDVLSYVNGLVGKGVDADGWYGTQCMDLTVDVMQRFFGWRPYGNAIALVDQPLPAGFQRIRTTSSTQIKAGDVMIWGLGYYAQYGHTGIATEDGRADGTFVSVDQNWINPSLEVGSPAAAIHHNMDGVWGVIRPPYEAASTPKPPAPKPDKPNLGQ-KVKPPAQKAVGKSASKITVGSKAPYNLKWSKGAYFNAKIDGLGATSATRYGDNRTNYRFDVGQAVYAPGTLIYVFEIIDGWCRIYWNNHNEWIWHERLIVKEVF