Detail of GM0020


The genetically modified enzybiotic, named Lys170-87 , constructed by Domains Assembly strategy, enhance the solubility,change or extend lytic spectrum.
With  Lys170-87
  Sequence Length:  286 AA.
  Mass:  30879.6 Da.
  Isoelectric Point:  7.81
  Function:  very effective in lysing S. aureus clinical isolates,Lys168-87 again showed a better lytic performance.


GM0020, constructed by Domains Assembly strategy.
    No schema construted on GM0020
GM0020[1-182]: derive from protein A8E261 sequence 1-182

A8E261, 289 AA., the Putative N-acetylmuramoyl-L-alanine amidase from Enterococcus phage phiEF24C (Enterococcus bacterio

Source: Enterococcus phage phiEF24C (Enterococcus bacterio

Domains and repeats

18-168:  IPR002502, the N-acetylmuramoyl-L-alanine amidase domain

GO term prediction

Biological Process:  0009253, the peptidoglycan catabolic process
Molecular Function:  0008745, the N-acetylmuramoyl-L-alanine amidase activity?

Linking to UniprotKB
Linging to InterPro
GM0020[183-287]: derive from protein U5T1I9 sequence 148-251

U5T1I9, 251 AA., the Phage lysin, N-acetylmuramoyl-L-alanine amidase from Staphylococcus aureus subsp. aureus Z172

Source: Staphylococcus aureus subsp. aureus Z172

Domains and repeats

4-133:  IPR007921, the CHAP domain

GO term prediction

    No GO info found on GM0020

Linking to UniprotKB
Linging to InterPro



: EAD, Amidase domain, derived from Enterococcus faecalis phage F170/08 endolysins Lys170;
: CBD, Cell wall-targeting domain, derived from staphylococcal phage endolysin Lys87;
Domain and repeats:
: IPR002502,N-acetylmuramoyl-L-alanine amidase domain;
GO term prediction:
0008745, the N-acetylmuramoyl-L-alanine amidase activity
0009253, the peptidoglycan catabolic process
  BlastP to GMEnzy


  1.  Expressed by pIVEX2.3d in E. coli XL1-Blue,  Purified by affinity chromatography using HisTrapTM HP columns (GE Healthcare) coupled to an KTA-Prime system(GEHealthcare).


    No activity data found on GM0020


  1. Fernandes, S.Proenca, D.Cantante, C.Silva, F. A.Leandro, C.Lourenco, S.Milheirico, Lencastre, H. (2012) Novel chimerical endolysins with broad antimicrobial activity against methicillin-resistant Staphylococcus aureus. . 03:333-343. [doi:10.1089/mdr.2012.0025] [PMID:22432707]


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