Detail of GM0023
Summary
The genetically modified enzybiotic, named PRF-119 , constructed by Domains Assembly strategy, improve the lytic activity.With PRF-119
Sequence Length: 191 AA.
Mass: 21480.9 Da.
Isoelectric Point: 7.73
Function: the recombinant endolysin PRF-119 was highly active against all S. aureus isolates tested, including MRSA isolates, while CoNS isolates from clinical collections were not susceptible.
Construction
GM0023, constructed by Domains Assembly strategy. No schema construted on GM0023
Details:
GM0023[1-126]: derive from protein I6X5A8 sequence 26-160
I6X5A8, 495 AA., the LysK from Staphylococcus phage Fi200W
Source: Staphylococcus phage Fi200W
196-369: IPR002502, the N-acetylmuramoyl-L-alanine amidase domain
409-481: IPR003646, the SH3-like domain, bacterial-type?
Linking to UniprotKB
Linging to InterPro
Domains and repeats
29-160: IPR007921, the CHAP domain196-369: IPR002502, the N-acetylmuramoyl-L-alanine amidase domain
409-481: IPR003646, the SH3-like domain, bacterial-type?
GO term prediction
Molecular Function: 0008745, the N-acetylmuramoyl-L-alanine amidase activityLinking to UniprotKB
Linging to InterPro
GM0023[127-191]: derive from protein P10547 sequence 411-485
P10547, 493 AA., the Lysostaphin from Staphylococcus simulans
Annotation
1-126
127-191
1-126
: EAD, CHAP domain, derived from endolysin of phageK;127-191
: CBD, SH3b domain, derived from lysostaphin;Domain and repeats:
127-187
: IPR003646,SH3-like domain, bacterial-type; 4-126
: IPR007921,CHAP domain; GO term prediction:
SFGVMEAGAIDADGYYHAQCQDLITDYVLWLTDNKVRTWGNAKDQIKQSYGTGFKIHENKPSTVPKKGWIAVFTSGSYEQWGHIGIVYDGGNTSTFTILEQNWNGYANKKPTKRVDNYYGLTHFIE-YKSESASFTPNTDIITRTTGPFRSMPQSGVLKAGQTIHYDEVMKQDGHVWVGYTGNSGQRIYLPV
Production
1. Expressed by pQE60 in E. coli XL1-Blue, Purified by ion-exchange and size exclusion chromatographyActivity
1. MIC test on Staphylococcus aureus (398 methicillin-susceptible Staphylococcus aureus ) by microdilution broth methodshowed MIC50 0.098μg/ml,MIC90 0.391μg/ml 2. MIC test on Staphylococcus aureus (776 methicillin-resistant S. aureus isolates) by microdilution broth method
showed MIC50 0.391μg/ml,MIC90 0.391μg/ml
Reference
1. Idelevich, E. A.von Eiff, C.Friedrich, A. W.Iannelli, D.Xia, G.Peters, G.Peschel, A.Wanninger, I.Bec. (2011) In vitro activity against Staphylococcus aureus of a novel antimicrobial agent, PRF-119, a recombinant chimeric bacteriophage endolysin. Antimicrobial agents and chemotherapy. 09:4416-4419. [doi:10.1128/AAC.00217-11] [PMID:21746950] [FULL TEXT]Comments
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