Detail of GM0025

Summary

The genetically modified enzybiotic, named A-S391 , constructed by Truncation strategy, lose the activities of cell targeting.
With  A-S391
  Sequence Length:  391 AA.
  Mass:  43052.4 Da.
  Isoelectric Point:  10.04
  Function:  Zymogram activity show no zone of clearing

Construction

GM0025, constructed by Truncation strategy.
No schema construted on GM0025

Details:

GM0025[1-391]: derive from protein I6X5A8 sequence 105-495

I6X5A8, 495 AA., the LysK from Staphylococcus phage Fi200W

Source: Staphylococcus phage Fi200W

Domains and repeats

29-160:  IPR007921, the CHAP domain
196-369:  IPR002502, the N-acetylmuramoyl-L-alanine amidase domain
409-481:  IPR003646, the SH3-like domain, bacterial-type?

GO term prediction

Molecular Function: 0008745, the N-acetylmuramoyl-L-alanine amidase activity

Linking to UniprotKB
Linging to InterPro

Annotation

1-391

92-241

: EAD, amidase-2 domain, derived from phage endolysin LysK;

307-376

: CBD, SH3b domain, derived from phage endolysin LysK;
Domain and repeats:

305-377

: IPR003646,SH3-like domain, bacterial-type?;

92-265

: IPR002502,N-acetylmuramoyl-L-alanine amidase domain;
GO term prediction:
0008745, the N-acetylmuramoyl-L-alanine amidase activity
0009253, the peptidoglycan catabolic process

AVFTSGSYEQWGHIGIVYDGGNTSTFTILEQNWNGYANKKPTKRVDNYYGLTHFIEIPVKAGTTVKKETAKKSASKTPAPKKKATLKVSKNHINYTMDKRGKKPEGMVIHNDAGRSSGQQYENSLANAGYARYANGIAHYYGSEGYVWEAIDAKNQIAWHTGDGTGANSGNFRFAGIEVCQSMSASDAQFLKNEQAVFQFTAEKFKEWGLTPNRKTVRLHMEFVPTACPHRSMVLHTGFNPVTQGRPSQAIMNKLKDYFIKQIKNYMDKGTSSSTVVKDGKTSSASTPATRPVTGSWKKNQYGTWYKPENATFVNGNQPIVTRIGSPFLNAPVGGNLPAGATIVYDEVCIQAGHIWIGYNAYNGNRVYCPVRTCQGVPPNQIPGVAWGVFK

BlastP to GMEnzy

Production

1. Expressed by pQE60 in E. coli XL1-Blue, Purified by ion-exchange and size exclusion chromatography

Activity

No activity data found on GM0025

Reference

No reference found on GM0025

Comments

No comments found on GM0025

GMEnzy : A Genetically Modified Enzybiotic Database