Detail of GM0028
Summary
The genetically modified enzybiotic, named C313 , constructed by Truncation strategy, improve the lytic activity.With C313
Sequence Length: 313 AA.
Mass: 34773.7 Da.
Isoelectric Point: 9.65
Function: Zymogram activity show zone of clearing
Construction
GM0028, constructed by Truncation strategy. No schema construted on GM0028
Details:
GM0028[1-313]: derive from protein I6X5A8 sequence 1-313
I6X5A8, 495 AA., the LysK from Staphylococcus phage Fi200W
Source: Staphylococcus phage Fi200W
196-369: IPR002502, the N-acetylmuramoyl-L-alanine amidase domain
409-481: IPR003646, the SH3-like domain, bacterial-type?
Linking to UniprotKB
Linging to InterPro
Domains and repeats
29-160: IPR007921, the CHAP domain196-369: IPR002502, the N-acetylmuramoyl-L-alanine amidase domain
409-481: IPR003646, the SH3-like domain, bacterial-type?
GO term prediction
Molecular Function: 0008745, the N-acetylmuramoyl-L-alanine amidase activityLinking to UniprotKB
Linging to InterPro
Annotation
1-313
35-160
: EAD, CHAP domain, derived from phage endolysin LysK;Domain and repeats:
29-160
: IPR007921,CHAP domain; GO term prediction:
MAKTQAEINKRLDAYAKGTVDSPYRVKKATSYDPSFGVMEAGAIDADGYYHAQCQDLITDYVLWLTDNKVRTWGNAKDQIKQSYGTGFKIHENKPSTVPKKGWIAVFTSGSYEQWGHIGIVYDGGNTSTFTILEQNWNGYANKKPTKRVDNYYGLTHFIEIPVKAGTTVKKETAKKSASKTPAPKKKATLKVSKNHINYTMDKRGKKPEGMVIHNDAGRSSGQQYENSLANAGYARYANGIAHYYGSEGYVWEAIDAKNQIAWHTGDGTGANSGNFRFAGIEVCQSMSASDAQFLKNEQAVFQFTAEKFKEWG