Detail of GM0032
Summary
The genetically modified enzybiotic, named PBDKZ-KMV36C , constructed by Domains Assembly strategy, improve the lytic activity.With PBDKZ-KMV36C
Sequence Length: 242 AA.
Mass: 26905 Da.
Isoelectric Point: 5.88
Function: exhibits a threefold higher specific activity than the native catalytic domain (KMV36C)
Construction
GM0032, constructed by Domains Assembly strategy. No schema construted on GM0032
Details:
GM0032[1-83]: derive from protein Q8SD18 sequence 1-83
Q8SD18, 260 AA., the PHIKZ144 from Pseudomonas phage phiKZ
GM0032[84-255]: derive from protein Q0E627 sequence 737-898
Q0E627, 898 AA., the Putative internal virion protein from Pseudomonas phage LKD16
Source: Pseudomonas phage LKD16
Linking to UniprotKB
Linging to InterPro
Domains and repeats
713-878: IPR023347, the Lysozyme domain?GO term prediction
Molecular Function: 0003796, the lysozyme activityLinking to UniprotKB
Linging to InterPro
Annotation
1-83
84-255
163-245
: CBD, Cell wall-targeting domain, derived from Pseudomonas phage endolysin KZ144;1-162
: EAD, lysozyme like domain, derived from P. aeruginosa phage??KMV lysin KMV36C;Domain and repeats:
2-73
: IPR002477,Peptidoglycan binding-like; GO term prediction:
MKVLRKGDRGDEVCQLQTLLNLCGYDVGKPDGIFGNNTFNQVVKFQKDNCLDSDGIVGKNTWAELFSKYSPPIPYKTIPMMLKQDVFNWRKELAQFEAYRGEAYKDADGYSVGLGHYLGSGNAGAGTTVTPEQAAQWFAEDTDRALDQGVRLADELGVTNNASILGLAGMAFQMGEGRARQFRNTFQAIKDRNKEAFEAGVRNSKWYTQTPNRAEAFIKRMAPHFDTPSQIGVDWYSAATAE