Detail of GM0032


Summary

The genetically modified enzybiotic, named PBDKZ-KMV36C , constructed by Domains Assembly strategy, improve the lytic activity.
With  PBDKZ-KMV36C
  Sequence Length:  242 AA.
  Mass:  26905 Da.
  Isoelectric Point:  5.88
  Function:  exhibits a threefold higher specific activity than the native catalytic domain (KMV36C)

Construction

GM0032, constructed by Domains Assembly strategy.
    No schema construted on GM0032
Details:
GM0032[1-83]: derive from protein Q8SD18 sequence 1-83

Q8SD18, 260 AA., the PHIKZ144 from Pseudomonas phage phiKZ

Source: Pseudomonas phage phiKZ

Domains and repeats

2-73:  IPR002477, the Peptidoglycan binding-like
94-182:  IPR008258, the Lytic transglycosylase-like SLT domain
96-233:  IPR023346, the Lysozyme-like domain

GO term prediction

    No GO info found on GM0032

Linking to UniprotKB
Linging to InterPro
GM0032[84-255]: derive from protein Q0E627 sequence 737-898

Q0E627, 898 AA., the Putative internal virion protein from Pseudomonas phage LKD16

Source: Pseudomonas phage LKD16

Domains and repeats

713-878:  IPR023347, the Lysozyme domain?

GO term prediction

Molecular Function:  0003796, the lysozyme activity


Linking to UniprotKB
Linging to InterPro

Annotation

1-83
84-255

163-245
: CBD, Cell wall-targeting domain, derived from Pseudomonas phage endolysin KZ144;
1-162
: EAD, lysozyme like domain, derived from P. aeruginosa phage??KMV lysin KMV36C;
Domain and repeats:
2-73
: IPR002477,Peptidoglycan binding-like;
GO term prediction:
MKVLRKGDRGDEVCQLQTLLNLCGYDVGKPDGIFGNNTFNQVVKFQKDNCLDSDGIVGKNTWAELFSKYSPPIPYKTIPMMLKQDVFNWRKELAQFEAYRGEAYKDADGYSVGLGHYLGSGNAGAGTTVTPEQAAQWFAEDTDRALDQGVRLADELGVTNNASILGLAGMAFQMGEGRARQFRNTFQAIKDRNKEAFEAGVRNSKWYTQTPNRAEAFIKRMAPHFDTPSQIGVDWYSAATAE
  BlastP to GMEnzy

Production

  1.  Expressed by pEXP5-CT/TOPO in E. coli BL21 (DE3),  Purified by metal affinity chromatography (HisTrap)

Activity

    No activity data found on GM0032

Reference

  1. Briers, Y.Schmelcher, M.Loessner, M. J.Hendrix, J.Engelborghs, Y.Volckaert, G.Lavigne, R.. (2009) The high-affinity peptidoglycan binding domain of Pseudomonas phage endolysin KZ144. Biochemical and biophysical research communication. 02:187-191. [doi:10.1016/j.bbrc.2009.03.161] [PMID:19348786]

Comments

    No comments found on GM0032


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