Detail of GM0034

Summary

The genetically modified enzybiotic, named LysK297 , constructed by Truncation strategy, decrease the lytic activity.
With  LysK297
  Sequence Length:  357 AA.
  Mass:  39727.5 Da.
  Isoelectric Point:  9.84
  Function:  show faint activity in both the zymogram and turbidity reduction assays, but no detectable activity in either plate lysis or MIC assays

Construction

GM0034, constructed by Truncation strategy.
No schema construted on GM0034

Details:

GM0034[1-297]: derive from protein I6X5A8 sequence 1-297

I6X5A8, 495 AA., the LysK from Staphylococcus phage Fi200W

Source: Staphylococcus phage Fi200W

Domains and repeats

29-160:  IPR007921, the CHAP domain
196-369:  IPR002502, the N-acetylmuramoyl-L-alanine amidase domain
409-481:  IPR003646, the SH3-like domain, bacterial-type?

GO term prediction

Molecular Function: 0008745, the N-acetylmuramoyl-L-alanine amidase activity

Linking to UniprotKB
Linging to InterPro

Annotation

1-297

35-160

: EAD, CHAP domain, derived from staphylococcal bacteriophage endolysin LysK;

197-297

: EAD, Amidase domain, derived from staphylococcal bacteriophage endolysin LysK;
Domain and repeats:

29-160

: IPR007921,CHAP domain;
GO term prediction:

MAKTQAEINKRLDAYAKGTVDSPYRVKKATSYDPSFGVMEAGAIDADGYYHAQCQDLITDYVLWLTDNKVRTWGNAKDQIKQSYGTGFKIHENKPSTVPKKGWIAVFTSGSYEQWGHIGIVYDGGNTSTFTILEQNWNGYANKKPTKRVDNYYGLTHFIEIPVKAGTTVKKETAKKSASKTPAPKKKATLKVSKNHINYTMDKRGKKPEGMVIHNDAGRSSGQQYENSLANAGYARYANGIAHYYGSEGYVWEAIDAKNQIAWHTGDGTGANSGNFRFAGIEVCQSMSASDAQFLKNEQAVFQFTAEKFKEWGLTPNRKTVRLHMEFVPTACPHRSMVLHTGFNPVTQGRPSQAIMN

BlastP to GMEnzy

Production

1. Expressed by pET21a in E. coli BL21 (DE3), Purified by nickel chromatography purification

Activity

No activity data found on GM0034

Reference

1. Becker, S. C.Dong, S.Baker, J. R.Foster-Frey, J.Pritchard, D. G.Donovan, D. M.. (2009) LysK CHAP endopeptidase domain is required for lysis of live staphylococcal cells. FEMS microbiology letters. 01:52-60. [doi:10.1111/j.1574-6968.2009.01541.x] [PMID:19493008]

Comments

No comments found on GM0034

GMEnzy : A Genetically Modified Enzybiotic Database