Detail of GM0038
Summary
The genetically modified enzybiotic, named LysKdelta221-390 , constructed by Truncation strategy, keep the lytic activity.With LysKdelta221-390
Sequence Length: 327 AA.
Mass: 36111.8 Da.
Isoelectric Point: 10.06
Function: has near full-length LysK lytic activity,
Construction
GM0038, constructed by Truncation strategy. No schema construted on GM0038
Details:
GM0038[1-221]: derive from protein I6X5A8 sequence 1-221
I6X5A8, 495 AA., the LysK from Staphylococcus phage Fi200W
Source: Staphylococcus phage Fi200W
196-369: IPR002502, the N-acetylmuramoyl-L-alanine amidase domain
409-481: IPR003646, the SH3-like domain, bacterial-type?
Linking to UniprotKB
Linging to InterPro
Domains and repeats
29-160: IPR007921, the CHAP domain196-369: IPR002502, the N-acetylmuramoyl-L-alanine amidase domain
409-481: IPR003646, the SH3-like domain, bacterial-type?
GO term prediction
Molecular Function: 0008745, the N-acetylmuramoyl-L-alanine amidase activityLinking to UniprotKB
Linging to InterPro
GM0038[222-327]: derive from protein I6X5A8 sequence 390-495
I6X5A8, 495 AA., the LysK from Staphylococcus phage Fi200W
Source: Staphylococcus phage Fi200W
196-369: IPR002502, the N-acetylmuramoyl-L-alanine amidase domain
409-481: IPR003646, the SH3-like domain, bacterial-type?
Linking to UniprotKB
Linging to InterPro
Domains and repeats
29-160: IPR007921, the CHAP domain196-369: IPR002502, the N-acetylmuramoyl-L-alanine amidase domain
409-481: IPR003646, the SH3-like domain, bacterial-type?
GO term prediction
Molecular Function: 0008745, the N-acetylmuramoyl-L-alanine amidase activityLinking to UniprotKB
Linging to InterPro
Annotation
1-221
222-327
35-160
: EAD, CHAP domain, derived from staphylococcal bacteriophage endolysin LysK;240-306
: CBD, SH3b domain, derived from staphylococcal bacteriophage endolysin LysK;Domain and repeats:
241-313
: IPR003646,SH3-like domain, bacterial-type?; 29-160
: IPR007921,CHAP domain; GO term prediction:
MAKTQAEINKRLDAYAKGTVDSPYRVKKATSYDPSFGVMEAGAIDADGYYHAQCQDLITDYVLWLTDNKVRTWGNAKDQIKQSYGTGFKIHENKPSTVPKKGWIAVFTSGSYEQWGHIGIVYDGGNTSTFTILEQNWNGYANKKPTKRVDNYYGLTHFIEIPVKAGTTVKKETAKKSASKTPAPKKKATLKVSKNHINYTMDKRGKKPEGMVIHNDAGRSS-STPATRPVTGSWKKNQYGTWYKPENATFVNGNQPIVTRIGSPFLNAPVGGNLPAGATIVYDEVCIQAGHIWIGYNAYNGNRVYCPVRTCQGVPPNQIPGVAWGVFK
Production
1. Expressed by pET21a in E. coli BL21 (DE3), Purified by nickel chromatography purificationActivity
1. MIC test on Staphylococcus aureus (Staphylococcus aureus strain Newman) by plate lysis assays and the turbidity-reduction assaysshowed 1 μg (2.7 mM)/10μl