Detail of GM0038


Summary

The genetically modified enzybiotic, named LysKdelta221-390 , constructed by Truncation strategy, keep the lytic activity.
With  LysKdelta221-390
  Sequence Length:  327 AA.
  Mass:  36111.8 Da.
  Isoelectric Point:  10.06
  Function:  has near full-length LysK lytic activity,

Construction

GM0038, constructed by Truncation strategy.
    No schema construted on GM0038
Details:
GM0038[1-221]: derive from protein I6X5A8 sequence 1-221

I6X5A8, 495 AA., the LysK from Staphylococcus phage Fi200W

Source: Staphylococcus phage Fi200W

Domains and repeats

29-160:  IPR007921, the CHAP domain
196-369:  IPR002502, the N-acetylmuramoyl-L-alanine amidase domain
409-481:  IPR003646, the SH3-like domain, bacterial-type?

GO term prediction

Molecular Function:  0008745, the N-acetylmuramoyl-L-alanine amidase activity


Linking to UniprotKB
Linging to InterPro
GM0038[222-327]: derive from protein I6X5A8 sequence 390-495

I6X5A8, 495 AA., the LysK from Staphylococcus phage Fi200W

Source: Staphylococcus phage Fi200W

Domains and repeats

29-160:  IPR007921, the CHAP domain
196-369:  IPR002502, the N-acetylmuramoyl-L-alanine amidase domain
409-481:  IPR003646, the SH3-like domain, bacterial-type?

GO term prediction

Molecular Function:  0008745, the N-acetylmuramoyl-L-alanine amidase activity


Linking to UniprotKB
Linging to InterPro

Annotation

1-221
222-327

35-160
: EAD, CHAP domain, derived from staphylococcal bacteriophage endolysin LysK;
240-306
: CBD, SH3b domain, derived from staphylococcal bacteriophage endolysin LysK;
Domain and repeats:
241-313
: IPR003646,SH3-like domain, bacterial-type?;
29-160
: IPR007921,CHAP domain;
GO term prediction:
MAKTQAEINKRLDAYAKGTVDSPYRVKKATSYDPSFGVMEAGAIDADGYYHAQCQDLITDYVLWLTDNKVRTWGNAKDQIKQSYGTGFKIHENKPSTVPKKGWIAVFTSGSYEQWGHIGIVYDGGNTSTFTILEQNWNGYANKKPTKRVDNYYGLTHFIEIPVKAGTTVKKETAKKSASKTPAPKKKATLKVSKNHINYTMDKRGKKPEGMVIHNDAGRSS-STPATRPVTGSWKKNQYGTWYKPENATFVNGNQPIVTRIGSPFLNAPVGGNLPAGATIVYDEVCIQAGHIWIGYNAYNGNRVYCPVRTCQGVPPNQIPGVAWGVFK
  BlastP to GMEnzy

Production

  1.  Expressed by pET21a in E. coli BL21 (DE3),  Purified by nickel chromatography purification

Activity

  1.  MIC test on Staphylococcus aureus (Staphylococcus aureus strain Newman) by plate lysis assays and the turbidity-reduction assays
  showed 1 μg (2.7 mM)/10μl

Reference

  1. Becker, S. C.Dong, S.Baker, J. R.Foster-Frey, J.Pritchard, D. G.Donovan, D. M.. (2009) LysK CHAP endopeptidase domain is required for lysis of live staphylococcal cells. FEMS microbiology letters. 01:52-60. [doi:10.1111/j.1574-6968.2009.01541.x] [PMID:19493008]

Comments

    No comments found on GM0038


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