Detail of GM0041

Summary

The genetically modified enzybiotic, named lambdaSa2-ECC , constructed by Truncation strategy, improve the lytic activity.
With  lambdaSa2-ECC
  Sequence Length:  240 AA.
  Mass:  25995.9 Da.
  Isoelectric Point:  6.11
  Function:  has a higher activity than the full-length protein

Construction

GM0041, constructed by Truncation strategy.
No schema construted on GM0041

Details:

GM0041[1-240]: derive from protein C5WI88 sequence 1-240

C5WI88, 449 AA., the Putative prophage LambdaSa2, lysin from Streptococcus dysgalactiae subsp. equisimilis (str

Source: Streptococcus dysgalactiae subsp. equisimilis (str

Domains and repeats

5-145:  IPR008044, the Bacteriophage lysin
6-138:  IPR000064, the Endopeptidase, NLPC/P60 domain
154-192:  IPR013168, the Cpl-7 lysozyme, C-terminal?
198-236:  IPR013169, the Cpl-7 lysozyme, C-terminal?
270-399:  IPR002901, the Mannosyl-glycoprotein endo-beta-N-acetylglucosamid

GO term prediction

Biological Process: 0009253, the peptidoglycan catabolic process
Molecular Function: 0004040, the amidase activity?

Linking to UniprotKB
Linging to InterPro

Annotation

1-240

1-148

: EAD, Endopeptidase domain, derived from Group B streptococcal prophage endolysin ??Sa2;

196-234

: CBD, Cpl-7 domain, derived from Group B streptococcal prophage endolysin ??Sa2;
Domain and repeats:

154-192

: IPR013168,Cpl-7 lysozyme, C-terminal?;

198-236

: IPR013169,Cpl-7 lysozyme, C-terminal?;

5-145

: IPR008044,Bacteriophage lysin;

6-138

: IPR000064,Endopeptidase, NLPC/P60 domain;
GO term prediction:

MAIETEKAISWMVARQGAVSYSMDYRNGPSSYDCSSAIYYALMSAGTISAGWAVNTEYMHDWLIKNGYVLIAENQDWNSQRGDVVIWGLRGQSAGAGGHVVMFVDSDNIIHCNYANNGITINNYNQTAASAGWMYSYVYRLATPATTSTAGKSLDTLVKETLAGIYGNGDTRKSALGNQYEAVMAVINGKATTTQKSIDELAQEVLAGKHGSGEQRKLSLGSNYDAVQAKVNEMLKQPQV

BlastP to GMEnzy

Production

1. Expressed by pET21a in E. coli BL21 (DE3), Purified by nickel-nitrilotriacetic acid (Ni-NTA) Agarose(nickel matrix)

Activity

No activity data found on GM0041

Reference

1. Donovan, D. M.Foster-Frey, J.. (2008) LambdaSa2 prophage endolysin requires Cpl-7-binding domains and amidase-5 domain for antimicrobial lysis of streptococci. FEMS microbiology letters. 01:22-33. [doi:10.1111/j.1574-6968.2008.01287.x] [PMID:18673393]

Comments

No comments found on GM0041

GMEnzy : A Genetically Modified Enzybiotic Database