Detail of GM0042

Summary

The genetically modified enzybiotic, named lambdaSa2-CCG , constructed by Truncation strategy, decrease the lytic activity.
With  lambdaSa2-CCG
  Sequence Length:  310 AA.
  Mass:  33126.1 Da.
  Isoelectric Point:  6.53
  Function:  showed very little activity in the turbidity reduction assay

Construction

GM0042, constructed by Truncation strategy.
No schema construted on GM0042

Details:

GM0042[1-310]: derive from protein C5WI88 sequence 140-449

C5WI88, 449 AA., the Putative prophage LambdaSa2, lysin from Streptococcus dysgalactiae subsp. equisimilis (str

Source: Streptococcus dysgalactiae subsp. equisimilis (str

Domains and repeats

5-145:  IPR008044, the Bacteriophage lysin
6-138:  IPR000064, the Endopeptidase, NLPC/P60 domain
154-192:  IPR013168, the Cpl-7 lysozyme, C-terminal?
198-236:  IPR013169, the Cpl-7 lysozyme, C-terminal?
270-399:  IPR002901, the Mannosyl-glycoprotein endo-beta-N-acetylglucosamid

GO term prediction

Biological Process: 0009253, the peptidoglycan catabolic process
Molecular Function: 0004040, the amidase activity?

Linking to UniprotKB
Linging to InterPro

Annotation

1-310

12-50

: CBD, Cpl-7 domain, derived from Group B streptococcal prophage endolysin ??Sa2;

56-94

: CBD, Cpl-7 domain, derived from Group B streptococcal prophage endolysin ??Sa2;

140-336

: EAD, Glycosidase domain, derived from Group B streptococcal prophage endolysin ??Sa2;
Domain and repeats:

131-260

: IPR002901,Mannosyl-glycoprotein endo-beta-N-acetylglucosamid;

15-53

: IPR013168,Cpl-7 lysozyme, C-terminal?;

59-97

: IPR013169,Cpl-7 lysozyme, C-terminal?;
GO term prediction:
0004040, the amidase activity

RLATPATTSTAGKSLDTLVKETLAGIYGNGDTRKSALGNQYEAVMAVINGKATTTQKSIDELAQEVLAGKHGSGEQRKLSLGSNYDAVQAKVNEMLKQPQVAEQSPAVKQDGDLLFNGAVLKKAILDKILAKCKEHDILPSYAITVLHFEGLWGTSAVGKADNNWGGMTWTGKGERPSGVTVTQGTARPASEGGNYMHYASVDDFLTDWFYLLSADGSYKVSGAKTFSEAVKGMFKVGGAVYDYAASGYDNYIVGMASRLKAIEQENGPLNKYDKQTDISVGQSDKIDVVIDSLEITINGVTYTSTKKPV

BlastP to GMEnzy

Production

1. Expressed by pET21a in E. coli BL21 (DE3), Purified by nickel-nitrilotriacetic acid (Ni-NTA) Agarose(nickel matrix)

Activity

No activity data found on GM0042

Reference

1. Donovan, D. M.Foster-Frey, J.. (2008) LambdaSa2 prophage endolysin requires Cpl-7-binding domains and amidase-5 domain for antimicrobial lysis of streptococci. FEMS microbiology letters. 01:22-33. [doi:10.1111/j.1574-6968.2008.01287.x] [PMID:18673393]

Comments

No comments found on GM0042

GMEnzy : A Genetically Modified Enzybiotic Database