Detail of GM0046

Summary

The genetically modified enzybiotic, named LysgaYS258 , constructed by Truncation strategy, decrease the lytic activity.
With  LysgaYS258
  Sequence Length:  258 AA.
  Mass:  27955.9 Da.
  Isoelectric Point:  6.94
  Function:  has no lytic activity

Construction

GM0046, constructed by Truncation strategy.
No schema construted on GM0046

Details:

GM0046[1-258]: derive from protein Q65YV6 sequence 1-258

Q65YV6, 310 AA., the Lysin from Lactobacillus phage phigaY

Source: Lactobacillus phage phigaY

Domains and repeats

4-209: IPR013781, the Glycoside hydrolase, catalytic domain
235-305: IPR003646, the SH3-like domain, bacterial-type

GO term prediction

Biological Process:  0005975, the carbohydrate metabolic process
Biological Process:  0009253, the peptidoglycan catabolic proces
Biological Process:  0016998, the peptidoglycan catabolic proces
Molecular Function:  0003796, the lysozyme activity

Linking to UniprotKB
Linging to InterPro

Annotation

1-258

1-206

: EAD, domain, derived from Lactobacillus gasseri JCM 1131T phage ??gaY endolysin,LysgaY;

217-310

: CBD, SH3bgaY domain, derived from Lactobacillus gasseri JCM 1131T phage ??gaY endolysin,LysgaY;
Domain and repeats:

4-209

: IPR013781,Glycoside hydrolase, catalytic domain;
GO term prediction:
0005975, the carbohydrate metabolic process

MVEVAKRSYGVDVSSHNNGNYSGSKFAVVKVSEGLDYRNPKAQSQVSTARANSMLPMAYHYARFSGNSNVAIQEGNYAVTSAKAVGLEAGTYLACDYEQGSGNETRGDRETNTTAILSFLDTIVGAGYKPLLYSGAYLMRDKINTSRILAKYPNCLWVAAYPSGNGTAVSEPNFGYFPSMNGVAIWQFTDNWRGLNVDGNISLIDLKNDSKPVAQPSVAQSASEKTWTDVQGMTWHEEHGTFITGGAINLRWGANTQS

BlastP to GMEnzy

Production

1. Expressed by pET-3c in E. coli NovaBlue(DE4), Purified by His-bind kit (Novagen, Germany) and Sephacryl S-200 HR

Activity

No activity data found on GM0046

Reference

1. Sugahara, K.Yokoi, K. J.Nakamura, Y.Nishino, T.Yamakawa, A.Taketo, A.Kodaira, K.. (2007) Mutational and biochemical analyses of the endolysin Lys(gaY) encoded by the Lactobacillus gasseri JCM 1131T phage phi gaY. Gene. 00:41-52. [doi:10.1016/j.gene.2007.08.023] [PMID:17920212]

Comments

No comments found on GM0046

GMEnzy : A Genetically Modified Enzybiotic Database