Detail of GM0050
Summary
The genetically modified enzybiotic, named LysgaYL206 , constructed by Truncation strategy, decrease the lytic activity.With LysgaYL206
Sequence Length: 206 AA.
Mass: 22317.7 Da.
Isoelectric Point: 7.39
Function: has no lytic activity
Construction
GM0050, constructed by Truncation strategy. No schema construted on GM0050
Details:
GM0050[1-206]: derive from protein Q65YV6 sequence 1-206
Q65YV6, 310 AA., the Lysin from Lactobacillus phage phigaY
Source: Lactobacillus phage phigaY
235-305: IPR003646, the SH3-like domain, bacterial-type
Biological Process: 0009253, the peptidoglycan catabolic proces
Biological Process: 0016998, the peptidoglycan catabolic proces
Molecular Function: 0003796, the lysozyme activity
Linking to UniprotKB
Linging to InterPro
Domains and repeats
4-209: IPR013781, the Glycoside hydrolase, catalytic domain235-305: IPR003646, the SH3-like domain, bacterial-type
GO term prediction
Biological Process: 0005975, the carbohydrate metabolic processBiological Process: 0009253, the peptidoglycan catabolic proces
Biological Process: 0016998, the peptidoglycan catabolic proces
Molecular Function: 0003796, the lysozyme activity
Linking to UniprotKB
Linging to InterPro
Annotation
1-206
1-206
: EAD, domain, derived from Lactobacillus gasseri JCM 1131T phage ??gaY endolysin,LysgaY;217-310
: CBD, SH3bgaY domain, derived from Lactobacillus gasseri JCM 1131T phage ??gaY endolysin,LysgaY;Domain and repeats:
GO term prediction:
MVEVAKRSYGVDVSSHNNGNYSGSKFAVVKVSEGLDYRNPKAQSQVSTARANSMLPMAYHYARFSGNSNVAIQEGNYAVTSAKAVGLEAGTYLACDYEQGSGNETRGDRETNTTAILSFLDTIVGAGYKPLLYSGAYLMRDKINTSRILAKYPNCLWVAAYPSGNGTAVSEPNFGYFPSMNGVAIWQFTDNWRGLNVDGNISLIDL
Production
1. Expressed by pET-3c in E. coli NovaBlue(DE8), Purified by His-bind kit (Novagen, Germany) and Sephacryl S-200 HRActivity
No activity data found on GM0050Reference
1. Sugahara, K.Yokoi, K. J.Nakamura, Y.Nishino, T.Yamakawa, A.Taketo, A.Kodaira, K.. (2007) Mutational and biochemical analyses of the endolysin Lys(gaY) encoded by the Lactobacillus gasseri JCM 1131T phage phi gaY. Gene. 00:41-52. [doi:10.1016/j.gene.2007.08.023] [PMID:17920212]2. Croux, C.Ronda, C.Lopez, R.Garcia, J. L.. (1993) Interchange of functional domains switches enzyme specificity: construction of a chimeric pneumococcal-clostridial cell wall lytic enzyme. Molecular microbiology. 05:1019-1025. [doi:10.1111/j.1365-2958.1993.tb01231.x] [PMID:7934908]