Detail of GM0051


Summary

The genetically modified enzybiotic, named LysgaYG182 , constructed by Truncation strategy, decrease the lytic activity.
With  LysgaYG182
  Sequence Length:  182 AA.
  Mass:  19575.7 Da.
  Isoelectric Point:  8.57
  Function:  has no lytic activity

Construction

GM0051, constructed by Truncation strategy.
    No schema construted on GM0051
Details:
GM0051[1-182]: derive from protein Q65YV6 sequence 1-182

Q65YV6, 310 AA., the Lysin from Lactobacillus phage phigaY

Source: Lactobacillus phage phigaY

Domains and repeats

4-209:  IPR013781, the Glycoside hydrolase, catalytic domain
235-305:  IPR003646, the SH3-like domain, bacterial-type

GO term prediction

Biological Process:  0005975, the carbohydrate metabolic process
Biological Process:  0009253, the peptidoglycan catabolic proces
Biological Process:  0016998, the peptidoglycan catabolic proces
Molecular Function:  0003796, the lysozyme activity


Linking to UniprotKB
Linging to InterPro

Annotation

1-182

1-206
: EAD, domain, derived from Lactobacillus gasseri JCM 1131T phage ??gaY endolysin,LysgaY;
217-310
: CBD, SH3bgaY domain, derived from Lactobacillus gasseri JCM 1131T phage ??gaY endolysin,LysgaY;
Domain and repeats:
GO term prediction:
MVEVAKRSYGVDVSSHNNGNYSGSKFAVVKVSEGLDYRNPKAQSQVSTARANSMLPMAYHYARFSGNSNVAIQEGNYAVTSAKAVGLEAGTYLACDYEQGSGNETRGDRETNTTAILSFLDTIVGAGYKPLLYSGAYLMRDKINTSRILAKYPNCLWVAAYPSGNGTAVSEPNFGYFPSMNG
  BlastP to GMEnzy

Production

  1.  Expressed by pET-3c in E. coli NovaBlue(DE9),  Purified by (Amersham Biosciences K.K.)

Activity

    No activity data found on GM0051

Reference

  1. Sugahara, K.Yokoi, K. J.Nakamura, Y.Nishino, T.Yamakawa, A.Taketo, A.Kodaira, K.. (2007) Mutational and biochemical analyses of the endolysin Lys(gaY) encoded by the Lactobacillus gasseri JCM 1131T phage phi gaY. Gene. 00:41-52. [doi:10.1016/j.gene.2007.08.023] [PMID:17920212]
  2. Diaz, E.Lopez, R.Garcia, J. L.. (1990) Chimeric phage-bacterial enzymes: a clue to the modular evolution of genes. Proceedings of the National Academy of Sciences of. 020:8125-8129. [doi:10.1073/pnas.87.20.8125] [PMID:1978320] [FULL TEXT]

Comments

    No comments found on GM0051


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