Detail of GM0052
Summary
The genetically modified enzybiotic, named LysgaYR147 , constructed by Truncation strategy, decrease the lytic activity.With LysgaYR147
Sequence Length: 147 AA.
Mass: 15856.5 Da.
Isoelectric Point: 8.85
Function: has no lytic activity
Construction
GM0052, constructed by Truncation strategy. No schema construted on GM0052
Details:
GM0052[1-147]: derive from protein Q65YV6 sequence 1-147
Q65YV6, 310 AA., the Lysin from Lactobacillus phage phigaY
Source: Lactobacillus phage phigaY
235-305: IPR003646, the SH3-like domain, bacterial-type
Biological Process: 0009253, the peptidoglycan catabolic proces
Biological Process: 0016998, the peptidoglycan catabolic proces
Molecular Function: 0003796, the lysozyme activity
Linking to UniprotKB
Linging to InterPro
Domains and repeats
4-209: IPR013781, the Glycoside hydrolase, catalytic domain235-305: IPR003646, the SH3-like domain, bacterial-type
GO term prediction
Biological Process: 0005975, the carbohydrate metabolic processBiological Process: 0009253, the peptidoglycan catabolic proces
Biological Process: 0016998, the peptidoglycan catabolic proces
Molecular Function: 0003796, the lysozyme activity
Linking to UniprotKB
Linging to InterPro
Annotation
1-147
1-206
: EAD, domain, derived from Lactobacillus gasseri JCM 1131T phage ??gaY endolysin,LysgaY;217-310
: CBD, SH3bgaY domain, derived from Lactobacillus gasseri JCM 1131T phage ??gaY endolysin,LysgaY;Domain and repeats:
GO term prediction:
MVEVAKRSYGVDVSSHNNGNYSGSKFAVVKVSEGLDYRNPKAQSQVSTARANSMLPMAYHYARFSGNSNVAIQEGNYAVTSAKAVGLEAGTYLACDYEQGSGNETRGDRETNTTAILSFLDTIVGAGYKPLLYSGAYLMRDKINTSR
Production
1. Expressed by pUC119 in E. coli NovaBlue(DE10), Purified by His-bind kit (Novagen, Germany) and Sephacryl S-200 HRActivity
No activity data found on GM0052Reference
1. Sugahara, K.Yokoi, K. J.Nakamura, Y.Nishino, T.Yamakawa, A.Taketo, A.Kodaira, K.. (2007) Mutational and biochemical analyses of the endolysin Lys(gaY) encoded by the Lactobacillus gasseri JCM 1131T phage phi gaY. Gene. 00:41-52. [doi:10.1016/j.gene.2007.08.023] [PMID:17920212]2. Diaz, E.Lopez, R.Garcia, J. L.. (1990) Chimeric phage-bacterial enzymes: a clue to the modular evolution of genes. Proceedings of the National Academy of Sciences of. 020:8125-8129. [doi:10.1073/pnas.87.20.8125] [PMID:1978320] [FULL TEXT]