Detail of GM0055


Summary

The genetically modified enzybiotic, named HSH3bgaY , constructed by Truncation strategy, keep the activities of cell targeting.
With  HSH3bgaY
  Sequence Length:  112 AA.
  Mass:  12452.7 Da.
  Isoelectric Point:  9.06
  Function:  bound to Gram-positive bacteria susceptible to LysgaY

Construction

GM0055, constructed by Truncation strategy.
    No schema construted on GM0055
Details:
GM0055[7-100]: derive from protein Q65YV6 sequence 217-310

Q65YV6, 310 AA., the Lysin from Lactobacillus phage phigaY

Source: Lactobacillus phage phigaY

Domains and repeats

4-209:  IPR013781, the Glycoside hydrolase, catalytic domain
235-305:  IPR003646, the SH3-like domain, bacterial-type

GO term prediction

Biological Process:  0005975, the carbohydrate metabolic process
Biological Process:  0009253, the peptidoglycan catabolic proces
Biological Process:  0016998, the peptidoglycan catabolic proces
Molecular Function:  0003796, the lysozyme activity


Linking to UniprotKB
Linging to InterPro

Annotation

7-100

1-206
: EAD, domain, derived from Lactobacillus gasseri JCM 1131T phage ??gaY endolysin,LysgaY;
217-310
: CBD, SH3bgaY domain, derived from Lactobacillus gasseri JCM 1131T phage ??gaY endolysin,LysgaY;
Domain and repeats:
25-95
: IPR003646,SH3-like domain, bacterial-type;
GO term prediction:
HHHHHHDLKNDSKPVAQPSVAQSASEKTWTDVQGMTWHEEHGTFITGGAINLRWGANTQSTLITTLPAGSEVKYNAWARDSAGRVWLQQPRENGKNGYLVGRVGSEPWGTFK
  BlastP to GMEnzy

Production

  1.  Expressed by pET-15b in E. coli NovaBlue(DE13),  Purified by His-bind kit (Novagen, Germany) and Sephacryl S-200 HR

Activity

    No activity data found on GM0055

Reference

  1. Sugahara, K.Yokoi, K. J.Nakamura, Y.Nishino, T.Yamakawa, A.Taketo, A.Kodaira, K.. (2007) Mutational and biochemical analyses of the endolysin Lys(gaY) encoded by the Lactobacillus gasseri JCM 1131T phage phi gaY. Gene. 00:41-52. [doi:10.1016/j.gene.2007.08.023] [PMID:17920212]
  2. Sheehan, M. M.Garcia, J. L.Lopez, R.Garcia, P.. (1996) Analysis of the catalytic domain of the lysin of the lactococcal bacteriophage Tuc2009 by chimeric gene assembling. FEMS microbiology letters. 01:23-28. [doi:10.1111/j.1574-6968.1996.tb08309.x] [PMID:8666197]

Comments

    No comments found on GM0055


CAPTCHA Image   Reload Image
Enter Code*: