Detail of GM0056

Summary

The genetically modified enzybiotic, named HSH3bgaYG , constructed by Truncation strategy, lose the activities of cell targeting.
With  HSH3bgaYG
  Sequence Length:  112 AA.
  Mass:  12323.5 Da.
  Isoelectric Point:  9.06
  Function:  lost the activities of cell targeting

Construction

GM0056, constructed by Truncation strategy.
No schema construted on GM0056

Details:

GM0056[7-100]: derive from protein Q65YV6 sequence 217-310

Q65YV6, 310 AA., the Lysin from Lactobacillus phage phigaY

Source: Lactobacillus phage phigaY

Domains and repeats

4-209: IPR013781, the Glycoside hydrolase, catalytic domain
235-305: IPR003646, the SH3-like domain, bacterial-type

GO term prediction

Biological Process:  0005975, the carbohydrate metabolic process
Biological Process:  0009253, the peptidoglycan catabolic proces
Biological Process:  0016998, the peptidoglycan catabolic proces
Molecular Function:  0003796, the lysozyme activity

Linking to UniprotKB
Linging to InterPro

Annotation

7-100

1-206

: EAD, domain, derived from Lactobacillus gasseri JCM 1131T phage ??gaY endolysin,LysgaY;

217-310

: CBD, SH3bgaY domain, derived from Lactobacillus gasseri JCM 1131T phage ??gaY endolysin,LysgaY;
Domain and repeats:

25-95

: IPR003646,SH3-like domain, bacterial-type;
GO term prediction:

HHHHHHDLKNDSKPVAQPSVAQSASEKTWTDVQGMTWHEEHGTFITGGAINLRWGANTQSTLITTLPAGSEVKYNAWARDSAGRVGLQQPRENGKNGYLVGRVGSEPWGTFK

BlastP to GMEnzy

Production

1. Expressed by pET-15b in E. coli NovaBlue(DE14), Purified by His-bind kit (Novagen, Germany) and Sephacryl S-200 HR

Activity

No activity data found on GM0056

Reference

1. Sugahara, K.Yokoi, K. J.Nakamura, Y.Nishino, T.Yamakawa, A.Taketo, A.Kodaira, K.. (2007) Mutational and biochemical analyses of the endolysin Lys(gaY) encoded by the Lactobacillus gasseri JCM 1131T phage phi gaY. Gene. 00:41-52. [doi:10.1016/j.gene.2007.08.023] [PMID:17920212]

Comments

No comments found on GM0056

GMEnzy : A Genetically Modified Enzybiotic Database