Detail of GM0057

Summary

The genetically modified enzybiotic, named HLg1233 , constructed by Truncation strategy, keep the activities of cell targeting.
With  HLg1233
  Sequence Length:  316 AA.
  Mass:  34422.1 Da.
  Isoelectric Point:  9.09
  Function:  bound to Gram-positive bacteria susceptible to LysgaY

Construction

GM0057, constructed by Truncation strategy.
No schema construted on GM0057

Details:

GM0057[7-317]: derive from protein Q65YV6 sequence 1-310

Q65YV6, 310 AA., the Lysin from Lactobacillus phage phigaY

Source: Lactobacillus phage phigaY

Domains and repeats

4-209: IPR013781, the Glycoside hydrolase, catalytic domain
235-305: IPR003646, the SH3-like domain, bacterial-type

GO term prediction

Biological Process:  0005975, the carbohydrate metabolic process
Biological Process:  0009253, the peptidoglycan catabolic proces
Biological Process:  0016998, the peptidoglycan catabolic proces
Molecular Function:  0003796, the lysozyme activity

Linking to UniprotKB
Linging to InterPro

Annotation

7-317

: MUT, mutation with D12A ;

: MUT, mutation with E33A ;

1-206

: EAD, domain, derived from Lactobacillus gasseri JCM 1131T phage ??gaY endolysin,LysgaY;

217-310

: CBD, SH3bgaY domain, derived from Lactobacillus gasseri JCM 1131T phage ??gaY endolysin,LysgaY;
Domain and repeats:

10-216

: IPR013781,Glycoside hydrolase, catalytic domain;

241-312

: IPR003646,SH3-like domain, bacterial-type;
GO term prediction:
0005975, the carbohydrate metabolic process

HHHHHHMVEVAKRSYGVAVSSHNNGNYSGSKFAVVKVSAGLDYRNPKAQSQVSTARANSMLPMAYHYARFSGNSNVAIQEGNYAVTSAKAVGLEAGTYLACDYEQGSGNETRGDRETNTTAILSFLDTIVGAGYKPLLYSGAYLMRDKINTSRILAKYPNCLWVAAYPSGNGTAVSEPNFGYFPSMNGVAIWQFTDNWRGLNVDGNISLIDLKNDSKPVAQPSVAQSASEKTWTDVQGMTWHEEHGTFITGGAINLRWGANTQSTLITTLPAGSEVKYNAWARDSAGRVWLQQPRENGKNGYLVGRVGSEPWGTFK

BlastP to GMEnzy

Production

1. Expressed by pET-15b in E. coli NovaBlue(DE15), Purified by His-bind kit (Novagen, Germany) and Sephacryl S-200 HR

Activity

No activity data found on GM0057

Reference

1. Sugahara, K.Yokoi, K. J.Nakamura, Y.Nishino, T.Yamakawa, A.Taketo, A.Kodaira, K.. (2007) Mutational and biochemical analyses of the endolysin Lys(gaY) encoded by the Lactobacillus gasseri JCM 1131T phage phi gaY. Gene. 00:41-52. [doi:10.1016/j.gene.2007.08.023] [PMID:17920212]

Comments

No comments found on GM0057

GMEnzy : A Genetically Modified Enzybiotic Database