Detail of GM0060

Summary

The genetically modified enzybiotic, named Lg1-2 , constructed by Mutagenesis strategy, decrease the lytic activity.
With  Lg1-2
  Sequence Length:  310 AA.
  Mass:  33698.3 Da.
  Isoelectric Point:  8.84
  Function:  decrease the lytic activity

Construction

GM0060, constructed by Mutagenesis strategy.
No schema construted on GM0060

Details:

GM0060[1-310]: derive from protein Q65YV6 sequence 1-310

Q65YV6, 310 AA., the Lysin from Lactobacillus phage phigaY

Source: Lactobacillus phage phigaY

Domains and repeats

4-209: IPR013781, the Glycoside hydrolase, catalytic domain
235-305: IPR003646, the SH3-like domain, bacterial-type

GO term prediction

Biological Process:  0005975, the carbohydrate metabolic process
Biological Process:  0009253, the peptidoglycan catabolic proces
Biological Process:  0016998, the peptidoglycan catabolic proces
Molecular Function:  0003796, the lysozyme activity

Linking to UniprotKB
Linging to InterPro

Annotation

1-310

1-206

: EAD, domain, derived from Lactobacillus gasseri JCM 1131T phage ??gaY endolysin,LysgaY;

217-310

: CBD, SH3bgaY domain, derived from Lactobacillus gasseri JCM 1131T phage ??gaY endolysin,LysgaY;

: MUT, mutation with M1K ;
Domain and repeats:

235-305

: IPR003646,SH3-like domain, bacterial-type;

4-209

: IPR013781,Glycoside hydrolase, catalytic domain;
GO term prediction:
0005975, the carbohydrate metabolic process

KVEVAKRSYGVDVSSHNNGNYSGSKFAVVKVSEGLDYRNPKAQSQVSTARANSMLPMAYHYARFSGNSNVAIQEGNYAVTSAKAVGLEAGTYLACDYEQGSGNETRGDRETNTTAILSFLDTIVGAGYKPLLYSGAYLMRDKINTSRILAKYPNCLWVAAYPSGNGTAVSEPNFGYFPSMNGVAIWQFTDNWRGLNVDGNISLIDLKNDSKPVAQPSVAQSASEKTWTDVQGMTWHEEHGTFITGGAINLRWGANTQSTLITTLPAGSEVKYNAWARDSAGRVWLQQPRENGKNGYLVGRVGSEPWGTFK

BlastP to GMEnzy

Production

1. Expressed by pUC119 in E. coli MV1184 sus?, Purified by His-bind kit (Novagen, Germany) and Sephacryl S-200 HR

Activity

No activity data found on GM0060

Reference

1. Sugahara, K.Yokoi, K. J.Nakamura, Y.Nishino, T.Yamakawa, A.Taketo, A.Kodaira, K.. (2007) Mutational and biochemical analyses of the endolysin Lys(gaY) encoded by the Lactobacillus gasseri JCM 1131T phage phi gaY. Gene. 00:41-52. [doi:10.1016/j.gene.2007.08.023] [PMID:17920212]

Comments

No comments found on GM0060

GMEnzy : A Genetically Modified Enzybiotic Database