Detail of GM0065

Summary

The genetically modified enzybiotic, named Lg33 , constructed by Mutagenesis strategy, decrease the lytic activity.
With  Lg33
  Sequence Length:  310 AA.
  Mass:  33643.3 Da.
  Isoelectric Point:  8.85
  Function:  has no lytic activity

Construction

GM0065, constructed by Mutagenesis strategy.
No schema construted on GM0065

Details:

GM0065[1-310]: derive from protein Q65YV6 sequence 1-310

Q65YV6, 310 AA., the Lysin from Lactobacillus phage phigaY

Source: Lactobacillus phage phigaY

Domains and repeats

4-209: IPR013781, the Glycoside hydrolase, catalytic domain
235-305: IPR003646, the SH3-like domain, bacterial-type

GO term prediction

Biological Process:  0005975, the carbohydrate metabolic process
Biological Process:  0009253, the peptidoglycan catabolic proces
Biological Process:  0016998, the peptidoglycan catabolic proces
Molecular Function:  0003796, the lysozyme activity

Linking to UniprotKB
Linging to InterPro

Annotation

1-310

1-206

: EAD, domain, derived from Lactobacillus gasseri JCM 1131T phage ??gaY endolysin,LysgaY;

217-310

: CBD, SH3bgaY domain, derived from Lactobacillus gasseri JCM 1131T phage ??gaY endolysin,LysgaY;

: MUT, mutation with E33A ;
Domain and repeats:

235-305

: IPR003646,SH3-like domain, bacterial-type;

4-209

: IPR013781,Glycoside hydrolase, catalytic domain;
GO term prediction:
0005975, the carbohydrate metabolic process

MVEVAKRSYGVDVSSHNNGNYSGSKFAVVKVSAGLDYRNPKAQSQVSTARANSMLPMAYHYARFSGNSNVAIQEGNYAVTSAKAVGLEAGTYLACDYEQGSGNETRGDRETNTTAILSFLDTIVGAGYKPLLYSGAYLMRDKINTSRILAKYPNCLWVAAYPSGNGTAVSEPNFGYFPSMNGVAIWQFTDNWRGLNVDGNISLIDLKNDSKPVAQPSVAQSASEKTWTDVQGMTWHEEHGTFITGGAINLRWGANTQSTLITTLPAGSEVKYNAWARDSAGRVWLQQPRENGKNGYLVGRVGSEPWGTFK

BlastP to GMEnzy

Production

1. Expressed by pUC119 in E. coli MV1184 sus?, Purified by His-bind kit (Novagen, Germany) and Sephacryl S-200 HR

Activity

No activity data found on GM0065

Reference

1. Sugahara, K.Yokoi, K. J.Nakamura, Y.Nishino, T.Yamakawa, A.Taketo, A.Kodaira, K.. (2007) Mutational and biochemical analyses of the endolysin Lys(gaY) encoded by the Lactobacillus gasseri JCM 1131T phage phi gaY. Gene. 00:41-52. [doi:10.1016/j.gene.2007.08.023] [PMID:17920212]

Comments

No comments found on GM0065

GMEnzy : A Genetically Modified Enzybiotic Database