Detail of GM0100
Summary
The genetically modified enzybiotic, named ami/CBD , constructed by Truncation strategy, decrease the lytic activity.With ami/CBD
Sequence Length: 301 AA.
Mass: 33608.7 Da.
Isoelectric Point: 9.16
Function: decrease the lytic activity
Construction
GM0100, constructed by Truncation strategy. No schema construted on GM0100
Details:
GM0100[1-301]: derive from protein Q8SDS7 sequence 181-481
Q8SDS7, 481 AA., the Amidase from Staphylococcus phage phi11 (Bacteriophage phi-11)
Source: Staphylococcus phage phi11 (Bacteriophage phi-11)
188-338: IPR002502, the N-acetylmuramoyl-L-alanine amidase domain
395-466: IPR003646, the SH3-like domain, bacterial-type
Molecular Function: 0008745, the N-acetylmuramoyl-L-alanine amidase activity?
Linking to UniprotKB
Linging to InterPro
Domains and repeats
7-148: IPR007921, the CHAP domain188-338: IPR002502, the N-acetylmuramoyl-L-alanine amidase domain
395-466: IPR003646, the SH3-like domain, bacterial-type
GO term prediction
Biological Process: 0009253, the peptidoglycan catabolic processMolecular Function: 0008745, the N-acetylmuramoyl-L-alanine amidase activity?
Linking to UniprotKB
Linging to InterPro
Annotation
1-301
1-310
: EAD, Amidase domain, derived from S. aureus NCTC8325 bacteriophages ??17;: CBD, SH3b domain, derived from S. aureus NCTC8325 bacteriophages ??18;
Domain and repeats:
215-286
: IPR003646,SH3-like domain, bacterial-type; 8-158
: IPR002502,N-acetylmuramoyl-L-alanine amidase domain; GO term prediction:
0008745, the N-acetylmuramoyl-L-alanine amidase activity
0009253, the peptidoglycan catabolic process
LKIIKDVVKGYDLPKRGSNPKGIVIHNDAGSKGATAEAYRNGLVNAPLSRLEAGIAHSYVSGNTVWQALDESQVGWHTANQIGNKYYYGIEVCQSMGADNATFLKNEQATFQECARLLKKWGLPANRNTIRLHNEFTSTSCPHRSSVLHTGFDPVTRGLLPEDKRLQLKDYFIKQIRAYMDGKIPVATVSNESSASSNTVKPVASAWKRNKYGTYYMEESARFTNGNQPITVRKVGPFLSCPVGYQFQPGGYCDYTEVMLQDGHVWVGYTWEGQRYYLPIRTWNGSAPPNQILGDLWGEIS