Detail of GM0100


Summary

The genetically modified enzybiotic, named ami/CBD , constructed by Truncation strategy, decrease the lytic activity.
With  ami/CBD
  Sequence Length:  301 AA.
  Mass:  33608.7 Da.
  Isoelectric Point:  9.16
  Function:  decrease the lytic activity

Construction

GM0100, constructed by Truncation strategy.
    No schema construted on GM0100
Details:
GM0100[1-301]: derive from protein Q8SDS7 sequence 181-481

Q8SDS7, 481 AA., the Amidase from Staphylococcus phage phi11 (Bacteriophage phi-11)

Source: Staphylococcus phage phi11 (Bacteriophage phi-11)

Domains and repeats

7-148:  IPR007921, the CHAP domain
188-338:  IPR002502, the N-acetylmuramoyl-L-alanine amidase domain
395-466:  IPR003646, the SH3-like domain, bacterial-type

GO term prediction

Biological Process:  0009253, the peptidoglycan catabolic process
Molecular Function:  0008745, the N-acetylmuramoyl-L-alanine amidase activity?


Linking to UniprotKB
Linging to InterPro

Annotation

1-301

1-310
: EAD, Amidase domain, derived from S. aureus NCTC8325 bacteriophages ??17;
: CBD, SH3b domain, derived from S. aureus NCTC8325 bacteriophages ??18;
Domain and repeats:
215-286
: IPR003646,SH3-like domain, bacterial-type;
8-158
: IPR002502,N-acetylmuramoyl-L-alanine amidase domain;
GO term prediction:
0008745, the N-acetylmuramoyl-L-alanine amidase activity
0009253, the peptidoglycan catabolic process
LKIIKDVVKGYDLPKRGSNPKGIVIHNDAGSKGATAEAYRNGLVNAPLSRLEAGIAHSYVSGNTVWQALDESQVGWHTANQIGNKYYYGIEVCQSMGADNATFLKNEQATFQECARLLKKWGLPANRNTIRLHNEFTSTSCPHRSSVLHTGFDPVTRGLLPEDKRLQLKDYFIKQIRAYMDGKIPVATVSNESSASSNTVKPVASAWKRNKYGTYYMEESARFTNGNQPITVRKVGPFLSCPVGYQFQPGGYCDYTEVMLQDGHVWVGYTWEGQRYYLPIRTWNGSAPPNQILGDLWGEIS
  BlastP to GMEnzy

Production

  1.  Expressed by pET22b in E. coli BL21(DE3),  Purified by nickel-nitrilotriacetic acid affinity chromatography

Activity

    No activity data found on GM0100

Reference

  1. Sass, P.Bierbaum, G.. (2007) Lytic activity of recombinant bacteriophage phi11 and phi12 endolysins on whole cells and biofilms of Staphylococcus aureus. Applied and environmental microbiology. 01:347-352. [doi:10.1128/AEM.01616-06] [PMID:17085695] [FULL TEXT]

Comments

    No comments found on GM0100


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