Detail of GM0107
Summary
The genetically modified enzybiotic, named delta3N , constructed by Truncation strategy, lose the activities of cell targeting.With delta3N
Sequence Length: 68 AA.
Mass: 7535.4 Da.
Isoelectric Point: 6.73
Function: lose the binding activity
Construction
GM0107, constructed by Truncation strategy. No schema construted on GM0107
Details:
GM0107[1-35]: derive from protein Q8LTE6 sequence 156-190
Q8LTE6, 233 AA., the N-acetylmuramoyl-L-alanine amidase from Bacillus phage Gamma
Source: Bacillus phage Gamma
189 - 232: IPR021976, the N-acetylmuramoyl-l-alanine amidase
Molecular Function: 0008745, the N-acetylmuramoyl-L-alanine amidase activity
Linking to UniprotKB
Linging to InterPro
Domains and repeats
3 - 156: IPR002502, the N-acetylmuramoyl-L-alanine amidase domain189 - 232: IPR021976, the N-acetylmuramoyl-l-alanine amidase
GO term prediction
Biological Process: 0009253, the peptidoglycan catabolic processMolecular Function: 0008745, the N-acetylmuramoyl-L-alanine amidase activity
Linking to UniprotKB
Linging to InterPro
GM0107[36-68]: derive from protein Q8LTE6 sequence 201-233
Q8LTE6, 233 AA., the N-acetylmuramoyl-L-alanine amidase from Bacillus phage Gamma
Source: Bacillus phage Gamma
189 - 232: IPR021976, the N-acetylmuramoyl-l-alanine amidase
Molecular Function: 0008745, the N-acetylmuramoyl-L-alanine amidase activity
Linking to UniprotKB
Linging to InterPro
Domains and repeats
3 - 156: IPR002502, the N-acetylmuramoyl-L-alanine amidase domain189 - 232: IPR021976, the N-acetylmuramoyl-l-alanine amidase
GO term prediction
Biological Process: 0009253, the peptidoglycan catabolic processMolecular Function: 0008745, the N-acetylmuramoyl-L-alanine amidase activity
Linking to UniprotKB
Linging to InterPro
Annotation
1-35
36-68
1-71
: CBD, Cell wall-targeting domain, derived from B. anthracis ??Phage lysin PlyG (PlyGB (PlyG binding domain; residues 156?C233));Domain and repeats:
1-35
: IPR002502,N-acetylmuramoyl-L-alanine amidase domain; 1-35
: IPR021976,N-acetylmuramoyl-l-alanine amidase; 36-68
: IPR021976,N-acetylmuramoyl-l-alanine amidase; 36-68
: IPR002502,N-acetylmuramoyl-L-alanine amidase domain; GO term prediction:
0008745, the N-acetylmuramoyl-L-alanine amidase activity
0009253, the peptidoglycan catabolic process
NGNVATTSPTKQNIIQSGAFSPYETPDVMGALTSSDGLTYFISKPTSDAQLKAMKEYLDRKGWWYEVK