Detail of GM0115


Summary

The genetically modified enzybiotic, named PlyGBS90-8 , constructed by Truncation strategy, improve the lytic activity.
With  PlyGBS90-8
  Sequence Length:  138 AA.
  Mass:  15314.8 Da.
  Isoelectric Point:  4.29
  Function:  has a ∼18-fold higher specific activity than wild-type PlyGBS

Construction

GM0115, constructed by Truncation strategy.
    No schema construted on GM0115
Details:
GM0115[1-138]: derive from protein Q5MY96 sequence 1-138

Q5MY96, 443 AA., the Lysin PlyGBS from Streptococcus phage NCTC11261

Source: Streptococcus phage NCTC11261

Domains and repeats

1 - 131:  IPR007921, the CHAP domain
152 - 339:  IPR017853, the Glycoside hydrolase, superfamily
390 - 437:  IPR003646, the SH3-like domain, bacterial-type

GO term prediction

Biological Process:  0005975, the carbohydrate metabolic process
Biological Process:  0009253, the peptidoglycan catabolic process
Biological Process:  0016998, the cell wall macromolecule catabolic process
Molecular Function:  0003796, the lysozyme activity


Linking to UniprotKB
Linging to InterPro

Annotation

1-138

1-138
: EAD, Endopeptidase domain, derived from group B streptococci bacteriophage lytic enzyme PlyGBS;
Domain and repeats:
1-138
: IPR007921,CHAP domain;
1-138
: IPR017853,Glycoside hydrolase, superfamily;
1-138
: IPR003646,SH3-like domain, bacterial-type;
GO term prediction:
MATYQEYKSRSNGNAYDIDGSFGAQCWDGYADYCKYLGLPYANCTNTGYARDIWEQRHENGILNYFDEVEVMQAGDVAIFMVVDGVTPYSHVAIFDSDAGGGYGWFLGQNQGGANGAYNIVKIPYSATYPTAFRPKVF
  BlastP to GMEnzy

Production

  1.  Expressed by pET28a in E. coli BL21(DE3),  Purified by anion exchange chromatography

Activity

    No activity data found on GM0115

Reference

  1. Cheng, Q.Fischetti, V. A.. (2007) Mutagenesis of a bacteriophage lytic enzyme PlyGBS significantly increases its antibacterial activity against group B streptococci. Applied microbiology and biotechnology. 06:1284-1291. [doi:10.1007/s00253-006-0771-1] [PMID:17186236]

Comments

    No comments found on GM0115


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