Detail of GM0118


Summary

The genetically modified enzybiotic, named PlyGBS95(1-146+394-443) , constructed by Truncation strategy, improve the lytic activity.
With  PlyGBS95(1-146+394-443)
  Sequence Length:  241 AA.
  Mass:  27008.8 Da.
  Isoelectric Point:  4.67
  Function:  has a ∼27-fold higher specific activity than wild-type PlyGBS.

Construction

GM0118, constructed by Truncation strategy.
    No schema construted on GM0118
Details:
GM0118[1-146]: derive from protein Q5MY96 sequence 1-146

Q5MY96, 443 AA., the Lysin PlyGBS from Streptococcus phage NCTC11261

Source: Streptococcus phage NCTC11261

Domains and repeats

1 - 131:  IPR007921, the CHAP domain
152 - 339:  IPR017853, the Glycoside hydrolase, superfamily
390 - 437:  IPR003646, the SH3-like domain, bacterial-type

GO term prediction

Biological Process:  0005975, the carbohydrate metabolic process
Biological Process:  0009253, the peptidoglycan catabolic process
Biological Process:  0016998, the cell wall macromolecule catabolic process
Molecular Function:  0003796, the lysozyme activity


Linking to UniprotKB
Linging to InterPro
GM0118[147-241]: derive from protein Q5MY96 sequence 349-443

Q5MY96, 443 AA., the Lysin PlyGBS from Streptococcus phage NCTC11261

Source: Streptococcus phage NCTC11261

Domains and repeats

1 - 131:  IPR007921, the CHAP domain
152 - 339:  IPR017853, the Glycoside hydrolase, superfamily
390 - 437:  IPR003646, the SH3-like domain, bacterial-type

GO term prediction

Biological Process:  0005975, the carbohydrate metabolic process
Biological Process:  0009253, the peptidoglycan catabolic process
Biological Process:  0016998, the cell wall macromolecule catabolic process
Molecular Function:  0003796, the lysozyme activity


Linking to UniprotKB
Linging to InterPro

Annotation

1-146
147-241

: EAD, Amidase domain, derived from group B streptococci bacteriophage lytic enzyme PlyGBS;
1-96
: EAD, Endopeptidase domain, derived from group B streptococci bacteriophage lytic enzyme PlyGBS;
Domain and repeats:
1-146
: IPR003646,SH3-like domain, bacterial-type;
1-146
: IPR007921,CHAP domain;
1-146
: IPR017853,Glycoside hydrolase, superfamily;
147-241
: IPR017853,Glycoside hydrolase, superfamily;
147-241
: IPR003646,SH3-like domain, bacterial-type;
147-241
: IPR007921,CHAP domain;
GO term prediction:
MATYQEYKSRSNGNAYDIDGSFGAQCWDGYADYCKYLGLPYANCTNTGYARDIWEQRHENGILNYFDEVEVMQAGDVAIFMVVDGVTPYSHVAIFDSDAGGGYGWFLGQNQGGANGAYNIVKIPYSATYPTAFRPKVFKNAVTVTGKMDIPKVDKPQELTFYQKLATNTKLDNSNVPYYEATLSTDYYVESKPNASSADKEFIKAGTRVRVYEKVNGWSRINHPESAQWVEDSYLVNATDM
  BlastP to GMEnzy

Production

  1.  Expressed by pCQJ94 in E. coli BL21(DE3),  Purified by anion exchange chromatography

Activity

    No activity data found on GM0118

Reference

  1. Cheng, Q.Fischetti, V. A.. (2007) Mutagenesis of a bacteriophage lytic enzyme PlyGBS significantly increases its antibacterial activity against group B streptococci. Applied microbiology and biotechnology. 06:1284-1291. [doi:10.1007/s00253-006-0771-1] [PMID:17186236]

Comments

    No comments found on GM0118


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