Detail of GM0119


Summary

The genetically modified enzybiotic, named PlyGBS107(1-394) , constructed by Truncation strategy, improve the lytic activity.
With  PlyGBS107(1-394)
  Sequence Length:  394 AA.
  Mass:  44060.7 Da.
  Isoelectric Point:  4.57
  Function:  has a ∼2-3-fold higher specific activity than wild-type PlyGBS.

Construction

GM0119, constructed by Truncation strategy.
    No schema construted on GM0119
Details:
GM0119[1-394]: derive from protein Q5MY96 sequence 1-394

Q5MY96, 443 AA., the Lysin PlyGBS from Streptococcus phage NCTC11261

Source: Streptococcus phage NCTC11261

Domains and repeats

1 - 131:  IPR007921, the CHAP domain
152 - 339:  IPR017853, the Glycoside hydrolase, superfamily
390 - 437:  IPR003646, the SH3-like domain, bacterial-type

GO term prediction

Biological Process:  0005975, the carbohydrate metabolic process
Biological Process:  0009253, the peptidoglycan catabolic process
Biological Process:  0016998, the cell wall macromolecule catabolic process
Molecular Function:  0003796, the lysozyme activity


Linking to UniprotKB
Linging to InterPro

Annotation

1-394

: EAD, Amidase domain, derived from group B streptococci bacteriophage lytic enzyme PlyGBS;
1-394
: EAD, EndopeptidasE domain, derived from group B streptococci bacteriophage lytic enzyme PlyGBS;
Domain and repeats:
1-394
: IPR017853,Glycoside hydrolase, superfamily;
1-394
: IPR003646,SH3-like domain, bacterial-type;
1-394
: IPR007921,CHAP domain;
GO term prediction:
MATYQEYKSRSNGNAYDIDGSFGAQCWDGYADYCKYLGLPYANCTNTGYARDIWEQRHENGILNYFDEVEVMQAGDVAIFMVVDGVTPYSHVAIFDSDAGGGYGWFLGQNQGGANGAYNIVKIPYSATYPTAFRPKVFKNAVTVTGNIGLNKGDYFIDVSAYQQADLTTTCQQAGTTKTIIKVSESIAWLSDRHQQQANTSDPIGYYHFGRFGGDSALAQREADLFLSNLPSKKVSYLVIDYEDSASADKQANTNAVIAFMDKIASAGYKPIYYSYKPFTLNNIDYQKIIAKYPNSIWIAGYPDYEVRTEPLWEFFPSMDGVRWWQFTSVGVAGGLDKNIVLLADDSSKMDIPKVDKPQELTFYQKLATNTKLDNSNVPYYEATLSTDYYVESK
  BlastP to GMEnzy

Production

  1.  Expressed by pET28a in E. coli BL21(DE3),  Purified by anion exchange chromatography

Activity

    No activity data found on GM0119

Reference

  1. Cheng, Q.Fischetti, V. A.. (2007) Mutagenesis of a bacteriophage lytic enzyme PlyGBS significantly increases its antibacterial activity against group B streptococci. Applied microbiology and biotechnology. 06:1284-1291. [doi:10.1007/s00253-006-0771-1] [PMID:17186236]

Comments

    No comments found on GM0119


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