Detail of GM0130

Summary

The genetically modified enzybiotic, named pLW11-N114A , constructed by Mutagenesis strategy, decrease the lytic activity.
With  pLW11-N114A
  Sequence Length:  477 AA.
  Mass:  53940.4 Da.
  Isoelectric Point:  8.73
  Function:  decrease the lytic activity

Construction

GM0130, constructed by Mutagenesis strategy.
No schema construted on GM0130

Details:

GM0130[1-477]: derive from protein Q5NTY4? sequence 1-477

Q5NTY4?, 477 AA., the Lysin from Staphylococcus phage phiWMY

Source: Staphylococcus phage phiWMY

Domains and repeats

No sequence info found on GM0130

GO term prediction

No GO info found on GM0130

Linking to UniprotKB
Linging to InterPro

Annotation

1-477

: EAD, full length domain, derived from Staphylococcus warneri M phage ??WMY lysWMY;

114

: MUT, mutation with N114A ;
Domain and repeats:
GO term prediction:

MKTKAQAKSWINSKIGKGIDWDGMYGYQCMDEAVDYIHHVTDGKVTMWGNAIDAPKNNFQGLCTVYTNTPEFRPAYGDVIVWSYGTFATYGHIAIVVNPDPYGDLQYITVLEQAWNGNGIYKTEFATIRTHDYTGVSHFIRPKFADEVKETAKTVNKLSVQKKIVTPKNSVERIKNYVKTSGYINGEHYELYNRGHKPKGVVIHNTAGTASATQEGQRLTNMTFQQLANGVAHVYIDKNTIYETLPEDRIAWHVAQQYGNTEFYGIEVCGSRNTDKEQFLANEQVAFQEAARRLKSWGMRANRNTVRLHHTFSSTECPDMSMLLHTGYSMKNGKPTQDITNKCADYFMKQINAYIDGKQPTSTVVGSSSSNKLKAKNKDKSTGWNTNEYGTLWKKEHATFTCGVRQGIVTRTTGPFTSCPQAGVLYYGQSVNYDTVCKQDGYVWISWTTSDGYDVWMPIRTWDRSTDKVSEIWGTIS

BlastP to GMEnzy

Production

1. Expressed by pUC121 in E. coli XL1-Blue, Purified by Not disclosed

Activity

No activity data found on GM0130

Reference

1. Yokoi, K. J.Kawahigashi, N.Uchida, M.Sugahara, K.Shinohara, M.Kawasaki, K.Nakamura, S.Taketo, A.Koda. (2005) The two-component cell lysis genes holWMY and lysWMY of the Staphylococcus warneri M phage varphiWMY: cloning, sequencing, expression, and mutational analysis in Escherichia coli. Gene. 00:97-108. [doi:10.1016/j.gene.2005.03.006] [PMID:15848115]

Comments

No comments found on GM0130

GMEnzy : A Genetically Modified Enzybiotic Database