Detail of GM0138

Summary

The genetically modified enzybiotic, named pLWK3 , constructed by Truncation strategy, lose the lytic activity.
With  pLWK3
  Sequence Length:  176 AA.
  Mass:  19734 Da.
  Isoelectric Point:  9.55
  Function:  lose the lytic activity

Construction

GM0138, constructed by Truncation strategy.
No schema construted on GM0138

Details:

GM0138[1-176]: derive from protein Q5NTY4? sequence 256-420

Q5NTY4?, 477 AA., the Lysin from Staphylococcus phage phiWMY

Source: Staphylococcus phage phiWMY

Domains and repeats

No sequence info found on GM0138

GO term prediction

No GO info found on GM0138

Linking to UniprotKB
Linging to InterPro

Annotation

1-176

: EAD, Amidase domain, derived from Staphylococcus warneri M phage ??WMY lysWMY;

: CBD, SH3b domain, derived from Staphylococcus warneri M phage ??WMY lysWMY;
Domain and repeats:
GO term prediction:

QQYGNTEFYGIEVCGSRNTDKEQFLANEQVAFQEAARRLKSWGMRANRNTVRLHHTFSSTECPDMSMLLHTGYSMKNGKPTQDITNKCADYFMKQINAYIDGKQPTSTVVGSSSSNKLKAKNKDKSTGWNTNEYGTLWKKEHATFTCGVRQGIVTRTTGPFTSCPQAGVLYYGQSV

BlastP to GMEnzy

Production

1. Expressed by pUC129 in E. coli XL1-Blue, Purified by Not disclosed

Activity

No activity data found on GM0138

Reference

1. Yokoi, K. J.Kawahigashi, N.Uchida, M.Sugahara, K.Shinohara, M.Kawasaki, K.Nakamura, S.Taketo, A.Koda. (2005) The two-component cell lysis genes holWMY and lysWMY of the Staphylococcus warneri M phage varphiWMY: cloning, sequencing, expression, and mutational analysis in Escherichia coli. Gene. 00:97-108. [doi:10.1016/j.gene.2005.03.006] [PMID:15848115]

Comments

No comments found on GM0138

GMEnzy : A Genetically Modified Enzybiotic Database