Detail of GM0140


Summary

The genetically modified enzybiotic, named D2 , constructed by Truncation strategy, decrease the lytic activity.
With  D2
  Sequence Length:  210 AA.
  Mass:  22842.9 Da.
  Isoelectric Point:  10.47
  Function:  decrease the lytic activity while improve enzymatic activity than parent endolysin

Construction

GM0140, constructed by Truncation strategy.
    No schema construted on GM0140
Details:
GM0140[1-210]: derive from protein P11187 sequence 1-210

P11187, 258 AA., the Lysozyme EC=3.2.1.17 from Bacillus phage phi29

Source: Bacillus phage phi29

Domains and repeats

1 - 147:  IPR023346, the Lysozyme-like domain
162 - 207:  IPR018392, the LysM domain
213 - 258:  IPR018392, the LysM domain

GO term prediction

Biological Process:  0009253, the peptidoglycan catabolic process
Biological Process:  0016998, the cell wall macromolecule catabolic process
Molecular Function:  0003796, the Lysozyme activity


Linking to UniprotKB
Linging to InterPro

Annotation

1-210

1-210
: EAD, Endopeptidase domain, derived from Bacillus amyloliquefaciens phage endolysin;
: EAD, LysM domain, derived from Bacillus amyloliquefaciens phage endolysin;
Domain and repeats:
1-210
: IPR018392,LysM domain;
1-210
: IPR018392,LysM domain ;
1-210
: IPR023346,Lysozyme-like domain;
GO term prediction:
MQISQAGINLIKSFEGLQLKAYKAVPTEKHYTIGYGHYGSDVSPRQVITAKQAEDMLRDDVQAFVDGVNKALKVSVTQNQFDALVSFAYNVGLGAFRSSSLLEYLNEGRTALAAAEFPKWNKSGGKVYQGLINRRAQEQALFNSGTPKNVSRGTSSTKTTPKYKVKSGDNLTKIAKKHNTTVATLLKLNPSIKDPNMIRVGQTINVTGSG
  BlastP to GMEnzy

Production

  1.  Expressed by pET21d(+) in E. coli JM109(DE3),  Purified by HiTrap(Amersham Pharmacia Biotech)

Activity

    No activity data found on GM0140

Reference

  1. Morita, M.Tanji, Y.Orito, Y.Mizoguchi, K.Soejima, A.Unno, H.. (2001) Functional analysis of antibacterial activity of Bacillus amyloliquefaciens phage endolysin against Gram-negative bacteria. FEBS letters. 00:56-59. [doi:10.1016/S0014-5793(01)02587-X] [PMID:11434926]

Comments

    No comments found on GM0140


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