Detail of GM0149


Summary

The genetically modified enzybiotic, named Mur-cA , constructed by Domains Assembly strategy, decrease the lytic activity.
With  Mur-cA
  Sequence Length:  349 AA.
  Mass:  38261.8 Da.
  Isoelectric Point:  10.17
  Function:  decrease the lytic activity

Construction

GM0149, constructed by Domains Assembly strategy.
    No schema construted on GM0149
Details:
GM0149[1-209]: derive from protein Q9AQE1 sequence 1-209

Q9AQE1, 209 AA., the N-acetyl muramidase from Leuconostoc citreum

Source: Leuconostoc citreum

Domains and repeats

63-201:  IPR002901, the Mannosyl-glycoprotein endo-beta-N-acetylglucosamid

GO term prediction

Biological Process:  0009253, the peptidoglycan catabolic process
Molecular Function:  0004040, the amidase activity


Linking to UniprotKB
Linging to InterPro
GM0149[210-349]: derive from protein Q9CIT4? sequence 300-439

Q9CIT4?, 439 AA., the Probable N-acetylmuramidase EC=3.2.1.17 from Lactococcus lactis subsp. lactis (strain IL1403)

Source: Lactococcus lactis subsp. lactis (strain IL1403)

Domains and repeats

    No sequence info found on GM0149

GO term prediction

    No GO info found on GM0149

Linking to UniprotKB
Linging to InterPro

Annotation

1-209
210-349

1-209
: EAD, Glucosaminidase domain, derived from L. citreum 22R Mur protein;
210-349
: EAD, LysM domain, derived from Lactococcus lactis autolysin AcmA;
Domain and repeats:
63-201
: IPR002901,Mannosyl-glycoprotein endo-beta-N-acetylglucosamid;
GO term prediction:
0004040, the amidase activity
MAKRKRRGKKKIFRLNLWLWISSILVLAIGATFWSQTLIYNEENAQGQDVVRQQNIAWINQLAPYARLMQEKYGVIASISIAQAILESDWHTSTLSPQYNNLFGIKADAGQKSVVLPTQEFQNGEWVTIQGRFAAYDSWQESMKAHAELLYHGTSWNAAQYQHVLAAKDYQAAANALTKDGYATDPTYAEKLIAIIQTWHLSRFDVPQK-GNSAGTTTPTTSVTPAKPASQTTIKVKSGDTLWGLSVKYKTTIAQLKSWNHLNSDTIFIGQNLIVSQSAGSSSSSTGSSSASTSSTSNSSAASNTSIHKVVKGDTLWGLSQKSGSPIASIKAWNHLSSDTILIGQYLRIK
  BlastP to GMEnzy

Production

  1.  Expressed by pCYT1 in L. lactis NZ9000,  Purified by nickel-nitriloacetic acid (Ni-NTA) spin column(Qiagen)

Activity

    No activity data found on GM0149

Reference

  1. Cibik, R.Tailliez, P.Langella, P.Chapot-Chartier, M. P.. (2001) Identification of Mur, an atypical peptidoglycan hydrolase derived from Leuconostoc citreum. Applied and environmental microbiology. 02:858-864. [doi:10.1128/AEM.67.2.858-864.2001] [PMID:11157255] [FULL TEXT]

Comments

    No comments found on GM0149


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