Detail of GM0157
Summary
The genetically modified enzybiotic, named Mdc-hly , constructed by Fusion to Peptides strategy, change or extend lytic spectrum.With Mdc-hly
Sequence Length: 178 AA.
Mass: 19032.3 Da.
Isoelectric Point: 9.74
Function: The recombinant fusion protein Mdc–hly has improved in vitro antimicrobial activity and action spectrum compared to Mdc and hly.
Construction
GM0157, constructed by Fusion to Peptides strategy. No schema construted on GM0157
Details:
GM0157[1-33]: derive from protein Q95VE8 sequence 28-60
Q95VE8, 63 AA., the Cecropin from Musca domestica (House fly)
Source: Musca domestica (House fly)
Linking to UniprotKB
Linging to InterPro
Domains and repeats
28-60: PF00272?, the CecropinGO term prediction
Cellular Component: 0005576, the extracellular region?Linking to UniprotKB
Linging to InterPro
GM0157[49-178]: derive from protein P61626 sequence 19-148
P61626, 148 AA., the Lysozyme C from Homo sapiens (Human)?
Annotation
1-33
49-178
1-33
: PEP, cecropin,cecropin;49-178
: EAD, Lysozyme domain, derived from Human lysozyme;Domain and repeats:
1-33
: PF00272?,Cecropin; 49-177
: IPR023346,Lysozyme-like domain; GO term prediction:
KIGKKIERVQQHTRDATIQTIGVAGGAANVAATGGGGSGGGGSGGGGSKVFERCELARTLKRLGMDGYRGISLANWMCLAKWESGYNTRATNYNAGDRSTDYGIFQINSRYWCNDGKTPGAVNACHLSCSALLQDNIADAVACAKRVVRDPQGIRAWVAWRNRCQNRDVRQYVQGCGV
Production
1. Expressed by pET-32a in E. coli BL21 (DE3), Purified by immobilized-metal affinity chromatography according to the instruction of HisTrap kit (GE healthcareActivity
1. MIC test on Staphylococcus aureus (Staphylococcus aureus(S. aureus) ATCC25923) by the turbidity-reduction assaysshowed 3.12μM 2. MIC test on Staphylococcus epidermidis (Staphylococcus epidermidis(S. epidermidis) ATCC 12) by the turbidity-reduction assays
showed 6.25μM 3. MIC test on Clostridium sporogenes (Clostridium sporogenes (C. sporogenes) ATCC 11437) by the turbidity-reduction assays
showed 1.56μM 4. MIC test on Micrococcus luteus (Micrococcus lysodeikticus(M. lysodeikticus) CICC 2) by the turbidity-reduction assays
showed 3.12μM 5. MIC test on Escherichia coli (Escherichia coli (E. coli) ATCC25922") by the turbidity-reduction assays
showed 0.39μM 6. MIC test on Shigella flexneri (Shigella flexneri (S. flexneri) ATCC 12022) by the turbidity-reduction assays
showed 0.78μM 7. MIC test on Pseudomonas aeruginosa (Pseudomonas aeruginosa (P. aeruginosa)ATCC27853) by the turbidity-reduction assays
showed 6.25μM 8. MIC test on Salmonella paratyphi-B (Salmonella paratyphi-B (S. paratyphi-B) CICC 2149) by the turbidity-reduction assays
showed 1.56μM 9. MIC test on Enterobacter cloacae (Enterobacter cloacae (E. cloacae) CICC 21652) by the turbidity-reduction assays
showed 1.56μM 10. MBC test on Staphylococcus aureus (Staphylococcus aureus(S. aureus) ATCC25923) by the turbidity-reduction assays
showed 6.25μM 11. MBC test on Staphylococcus epidermidis (Staphylococcus epidermidis(S. epidermidis) ATCC 12) by the turbidity-reduction assays
showed 12.5μM 12. MBC test on Clostridium sporogenes (Clostridium sporogenes (C. sporogenes) ATCC 11437) by the turbidity-reduction assays
showed 3.12μM 13. MBC test on Micrococcus luteus (Micrococcus lysodeikticus(M. lysodeikticus) CICC 2) by the turbidity-reduction assays
showed 6.25μM 14. MBC test on Escherichia coli (Escherichia coli (E. coli) ATCC25922) by the turbidity-reduction assays
showed 0.39μM 15. MBC test on Shigella flexneri (Shigella flexneri (S. flexneri) ATCC 12022) by the turbidity-reduction assays
showed 1.56μM 16. MBC test on Pseudomonas aeruginosa (Pseudomonas aeruginosa (P. aeruginosa)ATCC27853) by the turbidity-reduction assays
showed 12.5μM 17. MBC test on Salmonella paratyphi-B (Salmonella paratyphi-B (S. paratyphi-B) CICC 2149) by the turbidity-reduction assays
showed 1.56μM 18. MBC test on Enterobacter cloacae (Enterobacter cloacae (E. cloacae) CICC 21652) by the turbidity-reduction assays
showed 3.12μM