Detail of GM0158
Summary
The genetically modified enzybiotic, named HLz , constructed by Fusion to Peptides strategy, change or extend lytic spectrum.With HLz
Sequence Length: 134 AA.
Mass: 14874.7 Da.
Isoelectric Point: 9.21
Function: The lytic activity of the fusion lysozyme against Micrococcus lysodeikticus was 80% of wild-type protein. But disply bactericidal activity against gram-negtive bacteria due to insertion of a hydrophobic pentapeptide into its C-terminus.
Construction
GM0158, constructed by Fusion to Peptides strategy. No schema construted on GM0158
Details:
GM0158[1-129]: derive from protein P00698 sequence 19-147
P00698, 147 AA., the Lysozyme C from Gallus gallus (Chicken)?
Source: Gallus gallus (Chicken)?
Linking to UniprotKB
Linging to InterPro
Domains and repeats
18-146: IPR023346, the Lysozyme-like domainGO term prediction
No GO info found on GM0158Linking to UniprotKB
Linging to InterPro
Annotation
1-129
1-129
: EAD, lysozyme domain, derived from hen egg white lysozyme;130-134
: PEP, peptide,peptide,FFVAP;Domain and repeats:
GO term prediction:
KVFGRCELAAAMKRHGLDNYRGYSLGNWVCAAKFESNFNTQATNRNTDGSTDYGILQINSRWWCNDGRTPGSRNLCNIPCSALLSSDITASVNCAKKIVSDGNGMNAWVAWRNRCKGTDVQAWIRGCRLFFVAP
Production
1. Expressed by pYG-100 in S. cereuisiae AH22, Purified by two steps of cation-exchange chromatography first on CM-Toyopearl 650 column (7.5 X 1.7 cm) and rechromatographed on a CM-Toyopearl 650 column (12 X 1.5 cm)Activity
1. MIC50 test on Escherichia coli (E.coli wild-type 3301) by the turbidity-reduction assaysshowed 2.5 ug /ml
Reference
1. Ibrahim, H. R. Yamada, M. Matsushita, K. Kobayashi, K. Kato, A.. (1994) Enhanced bactericidal action of lysozyme to Escherichia coli by inserting a hydrophobic pentapeptide into its C terminus. J Biol Chem. 2697:5059-5063 [PMID:8106483]2. Ibrahim, H. R. Yamada, M. Kobayashi, K. Kato, A.. (1992) Bactericidal action of lysozyme against gram-negative bacteria due to insertion of a hydrophobic pentapeptide into its C-terminus. Biosci Biotechnol Biochem. 568:1361-1363 [PMID:1368848]