Detail of GM0159

Summary

The genetically modified enzybiotic, named C162 , constructed by Truncation strategy, improve the lytic activity.
With  C162
  Sequence Length:  162 AA.
  Mass:  18288.4 Da.
  Isoelectric Point:  8.81
  Function:  Zymogram activity show zone of clearing

Construction

GM0159, constructed by Truncation strategy.
No schema construted on GM0159

Details:

GM0159[1-162]: derive from protein I6X5A8 sequence 1-162

I6X5A8, 495 AA., the LysK from Staphylococcus phage Fi200W

Source: Staphylococcus phage Fi200W

Domains and repeats

29-160:  IPR007921, the CHAP domain
196-369:  IPR002502, the N-acetylmuramoyl-L-alanine amidase domain
409-481:  IPR003646, the SH3-like domain, bacterial-type?

GO term prediction

Molecular Function: 0008745, the N-acetylmuramoyl-L-alanine amidase activity

Linking to UniprotKB
Linging to InterPro

Annotation

1-162

35-160

: EAD, CHAP domain, derived from phage endolysin LysK;

35-160

: EAD, CHAP domain, derived from phage endolysin LysK;
Domain and repeats:

29-160

: IPR007921,CHAP domain;
GO term prediction:

MAKTQAEINKRLDAYAKGTVDSPYRVKKATSYDPSFGVMEAGAIDADGYYHAQCQDLITDYVLWLTDNKVRTWGNAKDQIKQSYGTGFKIHENKPSTVPKKGWIAVFTSGSYEQWGHIGIVYDGGNTSTFTILEQNWNGYANKKPTKRVDNYYGLTHFIEIP

BlastP to GMEnzy

Production

1. Expressed by pQE60 in E. coli XL1-Blue, Purified by ion-exchange and size exclusion chromatography

Activity

No activity data found on GM0159

Reference

1. Yokoi, K. J.Kawahigashi, N.Uchida, M.Sugahara, K.Shinohara, M.Kawasaki, K.Nakamura, S.Taketo, A.Koda. (2005) The two-component cell lysis genes holWMY and lysWMY of the Staphylococcus warneri M phage varphiWMY: cloning, sequencing, expression, and mutational analysis in Escherichia coli. Gene. 00:97-108. [doi:10.1016/j.gene.2005.03.006] [PMID:15848115]

Comments

No comments found on GM0159

GMEnzy : A Genetically Modified Enzybiotic Database