Detail of GM0171


Summary

The genetically modified enzybiotic, named Cpl-7S , constructed by Mutagenesis strategy, improve the lytic activity.
With  Cpl-7S
  Sequence Length:  342 AA.
  Mass:  38516.5 Da.
  Isoelectric Point:  4.58
  Function:  a single 25-30 µg dose of Cpl-7S significantly increased the survival rate of zebrafish embryos,infected with S. pneumoniae or S. pyogenes, confirming the killing effect of Cpl-7S in vivo.

Construction

GM0171, constructed by Mutagenesis strategy.
P19385: CP-7 lysin
UniProt

IPR013781:3-185
IPR017853:3 - 185
IPR013168:205-246
IPR013168:205 - 246
IPR013168:253-294
IPR013168:253 - 294
IPR013168:301-342
IPR013168:301 - 342
InterPro

GM0171:1-188
GMEnzy

30
60
90
120
150
180
210
240
270
300
330
342

Details:
GM0171[1-188]: derive from protein P19385 sequence 1-342

P19385, 342 AA., the CP-7 lysin from Streptococcus phage Cp-7 (Bacteriophage Cp-7)

Source: Streptococcus phage Cp-7 (Bacteriophage Cp-7)

Domains and repeats

3-185:  IPR013781, the Glycoside hydrolase, catalytic domain
205-246:  IPR013168, the Cpl-7 lysozyme, C-terminal
253-294:  IPR013168, the Cpl-7 lysozyme, C-terminal
301-342:  IPR013168, the Cpl-7 lysozyme, C-terminal
3 - 185:  IPR017853, the Glycoside hydrolase, superfamily
205 - 246:  IPR013168, the Cpl-7 lysozyme, C-terminal
253 - 294:  IPR013168, the Cpl-7 lysozyme, C-terminal
301 - 342:  IPR013168, the Cpl-7 lysozyme, C-terminal

GO term prediction

Biological Process:  0009253, the peptidoglycan catabolic process
Biological Process:  0005975, the carbohydrate metabolic process
Biological Process:  0016998, the cell wall macromolecule catabolic process?
Molecular Function:  0003796, the lysozyme activity?
Biological Process:  0005975, the carbohydrate metabolic process?
Biological Process:  0009253, the peptidoglycan catabolic process?
Biological Process:  0016998, the cell wall macromolecule catabolic process
Molecular Function:  0003796, the lysozyme activity


Linking to UniprotKB
Linging to InterPro

Annotation

1-188
50
100
150
200
250
300
342

1-188
: EAD, Lysozyme domain, derived from CP-7 lysin;
205-338
: CBD, CPL-7 CBD domain, derived from CP-7 lysin;
15 AA
: MUT, mutation with inverting the charge of the cell wall-binding modu ;
Domain and repeats:
1-188
: IPR013168,Cpl-7 lysozyme, C-terminal ;
1-188
: IPR013168,Cpl-7 lysozyme, C-terminal;
1-188
: IPR013168,Cpl-7 lysozyme, C-terminal ;
1-188
: IPR017853,Glycoside hydrolase, superfamily;
205-92
: IPR013168,Cpl-7 lysozyme, C-terminal;
253-140
: IPR013168,Cpl-7 lysozyme, C-terminal;
3-31
: IPR013781,Glycoside hydrolase, catalytic domain;
301-188
: IPR013168,Cpl-7 lysozyme, C-terminal;
GO term prediction:
0005975, the carbohydrate metabolic process
MVKKNDLFVDVASHQGYDISGILEEAGTTNTIIKVSESTSYLNPCLSAQVSQSNPIGFYHFACFGGNEEEAEAEARYFLDNVPTQVKYLVLDYEDHASASVQRNTTACLRFMQMIAEAGYTPIYYSYKPFTLDNVDYQQILAQFPNSLWIAGYGLNDGTANFEYFPSMDGIRWWQYSSNPFDKNIVLLDDEKEDNINNENTLKSLTTVANEVIQGKWGNGQERYKSLANRGYNPQAVQNKVNEILNAREIADLTTVANEVIQGKWGNGQERYKSLANRGYNPQAVQNKVNEILNAREIADLTTVANEVIQGKWGNGQERYKSLANRGYNPQAVQNKVNELLS
  BlastP to GMEnzy

Production

  1.  Expressed by pUC-derivative plasmid in E. coli BL21(DE3),  Purified by DEAE cellulose ionic-exchange chromatography.

Activity

    No activity data found on GM0171

Reference

  1. Diez-Martinez, R.de Paz, H.Bustamante, N.Garcia, E.Menendez, M.Garcia, P.. (2013) Improved Lethal Effect of Cpl-7, a Pneumococcal Phage Lysozyme of Broad Bactericidal Activity by Inverting Net Charge of its Cell Wall-Binding Module.. Antimicrob Agents Chemothe. REF0046:. [doi:10.1128/AAC.01372-13] [PMID:23959317] [FULL TEXT]

Comments

    No comments found on GM0171


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