Detail of GM0171
Summary
The genetically modified enzybiotic, named Cpl-7S , constructed by Mutagenesis strategy, improve the lytic activity.With Cpl-7S
Sequence Length: 342 AA.
Mass: 38516.5 Da.
Isoelectric Point: 4.58
Function: a single 25-30 µg dose of Cpl-7S significantly increased the survival rate of zebrafish embryos,infected with S. pneumoniae or S. pyogenes, confirming the killing effect of Cpl-7S in vivo.
Construction
GM0171, constructed by Mutagenesis strategy. No schema construted on GM0171
Details:
GM0171[1-188]: derive from protein P19385 sequence 1-342
P19385, 342 AA., the CP-7 lysin from Streptococcus phage Cp-7 (Bacteriophage Cp-7)
Source: Streptococcus phage Cp-7 (Bacteriophage Cp-7)
205-246: IPR013168, the Cpl-7 lysozyme, C-terminal
253-294: IPR013168, the Cpl-7 lysozyme, C-terminal
301-342: IPR013168, the Cpl-7 lysozyme, C-terminal
3 - 185: IPR017853, the Glycoside hydrolase, superfamily
205 - 246: IPR013168, the Cpl-7 lysozyme, C-terminal
253 - 294: IPR013168, the Cpl-7 lysozyme, C-terminal
301 - 342: IPR013168, the Cpl-7 lysozyme, C-terminal
Biological Process: 0005975, the carbohydrate metabolic process
Biological Process: 0016998, the cell wall macromolecule catabolic process?
Molecular Function: 0003796, the lysozyme activity?
Biological Process: 0005975, the carbohydrate metabolic process?
Biological Process: 0009253, the peptidoglycan catabolic process?
Biological Process: 0016998, the cell wall macromolecule catabolic process
Molecular Function: 0003796, the lysozyme activity
Linking to UniprotKB
Linging to InterPro
Domains and repeats
3-185: IPR013781, the Glycoside hydrolase, catalytic domain205-246: IPR013168, the Cpl-7 lysozyme, C-terminal
253-294: IPR013168, the Cpl-7 lysozyme, C-terminal
301-342: IPR013168, the Cpl-7 lysozyme, C-terminal
3 - 185: IPR017853, the Glycoside hydrolase, superfamily
205 - 246: IPR013168, the Cpl-7 lysozyme, C-terminal
253 - 294: IPR013168, the Cpl-7 lysozyme, C-terminal
301 - 342: IPR013168, the Cpl-7 lysozyme, C-terminal
GO term prediction
Biological Process: 0009253, the peptidoglycan catabolic processBiological Process: 0005975, the carbohydrate metabolic process
Biological Process: 0016998, the cell wall macromolecule catabolic process?
Molecular Function: 0003796, the lysozyme activity?
Biological Process: 0005975, the carbohydrate metabolic process?
Biological Process: 0009253, the peptidoglycan catabolic process?
Biological Process: 0016998, the cell wall macromolecule catabolic process
Molecular Function: 0003796, the lysozyme activity
Linking to UniprotKB
Linging to InterPro
Annotation
1-188
1-188
: EAD, Lysozyme domain, derived from CP-7 lysin;205-338
: CBD, CPL-7 CBD domain, derived from CP-7 lysin;15 AA
: MUT, mutation with inverting the charge of the cell wall-binding modu ;Domain and repeats:
1-188
: IPR013168,Cpl-7 lysozyme, C-terminal ; 1-188
: IPR013168,Cpl-7 lysozyme, C-terminal; 1-188
: IPR013168,Cpl-7 lysozyme, C-terminal ; 1-188
: IPR017853,Glycoside hydrolase, superfamily; 205-92
: IPR013168,Cpl-7 lysozyme, C-terminal; 253-140
: IPR013168,Cpl-7 lysozyme, C-terminal; 3-31
: IPR013781,Glycoside hydrolase, catalytic domain; 301-188
: IPR013168,Cpl-7 lysozyme, C-terminal; GO term prediction:
0005975, the carbohydrate metabolic process
MVKKNDLFVDVASHQGYDISGILEEAGTTNTIIKVSESTSYLNPCLSAQVSQSNPIGFYHFACFGGNEEEAEAEARYFLDNVPTQVKYLVLDYEDHASASVQRNTTACLRFMQMIAEAGYTPIYYSYKPFTLDNVDYQQILAQFPNSLWIAGYGLNDGTANFEYFPSMDGIRWWQYSSNPFDKNIVLLDDEKEDNINNENTLKSLTTVANEVIQGKWGNGQERYKSLANRGYNPQAVQNKVNEILNAREIADLTTVANEVIQGKWGNGQERYKSLANRGYNPQAVQNKVNEILNAREIADLTTVANEVIQGKWGNGQERYKSLANRGYNPQAVQNKVNELLS