Detail of GM0178


Summary

The genetically modified enzybiotic, named EADPSA_CBD118_A1 , constructed by Domains Assembly strategy, change or extend lytic spectrum.
With  EADPSA_CBD118_A1
  Sequence Length:  338 AA.
  Mass:  36512.1 Da.
  Isoelectric Point:  10.34
  Function:  

Construction

GM0178, constructed by Domains Assembly strategy.
    No schema construted on GM0178
Details:
GM0178[1-185]: derive from protein Q8W5Y8 sequence 1-185

Q8W5Y8, 314 AA., the Ply protein from Listeria phage PSA

Source: Listeria phage PSA

Domains and repeats

    No sequence info found on GM0178

GO term prediction

    No GO info found on GM0178

Linking to UniprotKB
Linging to InterPro
GM0178[186-338]: derive from protein Q37976 sequence 129-281

Q37976, 281 AA., the L-alanyl-D-glutamate peptidase from Listeria phage A118 (Bacteriophage A118)

Source: Listeria phage A118 (Bacteriophage A118)

Domains and repeats

8-121:  IPR009045, the Hedgehog signalling/DD-peptidase zinc-binding doma
153-193:  IPR022016, the Domain of unknown function DUF3597

GO term prediction

    No GO info found on GM0178

Linking to UniprotKB
Linging to InterPro

Annotation

1-185
186-338

1-185
: EAD, N-acetylmuramoyl-L-alanine amidases domain, derived from L. monocytogenes PSA endolysin PlyPSA;
186-338
: CBD, CBD118 domain, derived from Listeria phage endolysin Ply118;
Domain and repeats:
210-250
: IPR022016,Domain of unknown function DUF3597;
GO term prediction:
MSNYSMSRGHSDKCVGAEDILSEIKEAEKVLNAASDELKREGHNVKTFIDRTSTTQSANLNKIVNWHNANPADVHISVHLNAGKGTGVEVWYYAGDEKGRKLAVEISAKMAKALGLPNRGAKATKDLRFLNSTKGTAVLLEVCFVDRKEDANAIHKSGMYDKLGIAIAEGLTGKTVAAKNPNRHS-DTFGKGASTSNSSKPSADTNTNSLGLVDYMNLNKLDSSFANRKKLATSYGIKNYSGTATQNTTLLAKLKAGKPHTPASKNTYYTENPRKVKTLVQCDLYKSVDFTTKNQTGGTFPPGTVFTISGMGKTKGGTPRLKTKSGYYLTANTKFVKKI
  BlastP to GMEnzy

Production

  1.  Expressed by pQE-30 in E. coli XL1-Blue MRF,  Purified by immobilized metal affinity chromatography (IMAC) with Ni-NTA Superflow resin (Qiagen) using Micro Biospin columns (Bio-Rad)

Activity

    No activity data found on GM0178

Reference

  1. Schmelcher M, Tchang VS, Loessner MJ. (2011) Domain shuffling and module engineering of Listeria phage endolysins for enhanced lytic activity and binding affinity.. Microb Biotechnol . REF0047:651-662. [doi:10.1111/j.1751-7915.2011.00263.x] [PMID:21535426] [FULL TEXT]

Comments

    No comments found on GM0178


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