Detail of GM0186

Summary

The genetically modified enzybiotic, named SAL-1D6 , constructed by Mutagenesis strategy, decrease the lytic activity.
With  SAL-1D6
  Sequence Length:  495 AA.
  Mass:  54770.5 Da.
  Isoelectric Point:  9.89
  Function:

Construction

GM0186, constructed by Mutagenesis strategy.
No schema construted on GM0186

Details:

GM0186[1-495]: derive from protein I6X5A8 sequence 1-495

I6X5A8, 495 AA., the LysK from Staphylococcus phage Fi200W

Source: Staphylococcus phage Fi200W

Domains and repeats

29-160:  IPR007921, the CHAP domain
196-369:  IPR002502, the N-acetylmuramoyl-L-alanine amidase domain
409-481:  IPR003646, the SH3-like domain, bacterial-type?

GO term prediction

Molecular Function: 0008745, the N-acetylmuramoyl-L-alanine amidase activity

Linking to UniprotKB
Linging to InterPro

Annotation

1-495

45-159

: EAD, CHAP domain, derived from S. aureus bacteriophage SAP-1 endolysin SAL-1;

207-335

: EAD, PGRR domain, derived from S. aureus bacteriophage SAP-1 endolysin SAL-1;

409-475

: CBD, SH3_5 domain, derived from S. aureus bacteriophage SAP-1 endolysin SAL-1;

: MUT, mutation with V26I ;

114

: MUT, mutation with Q114E ;

486

: MUT, mutation with I486Q ;
Domain and repeats:

196-369

: IPR002502,N-acetylmuramoyl-L-alanine amidase domain;

29-160

: IPR007921,CHAP domain;

409-481

: IPR003646,SH3-like domain, bacterial-type?;
GO term prediction:
0008745, the N-acetylmuramoyl-L-alanine amidase activity
0009253, the peptidoglycan catabolic process

MAKTQAEINKRLDAYAKGTVDSPYRIKKATSYDPSFGVMEAGAIDADGYYHAQCQDLITDYVLWLTDNKVRTWGNAKDQIKQSYGTGFKIHENKPSTVPKKGWIAVFTSGSYEEWGHIGIVYDGGNTSTFTILEQNWNGYANKKPTKRVDNYYGLTHFIEIPVKAGTTVKKETAKKSASKTPAPKKKATLKVSKNHINYTMDKRGKKPEGMVIHNDAGRSSGQQYENSLANAGYARYANGIAHYYGSEGYVWEAIDAKNQIAWHTGDGTGANSGNFRFAGIEVCQSMSASDAQFLKNEQAVFQFTAEKFKEWGLTPNRKTVRLHMEFVPTACPHRSMVLHTGFNPVTQGRPSQAIMNKLKDYFIKQIKNYMDKGTSSSTVVKDGKTSSASTPATRPVTGSWKKNQYGTWYKPENATFVNGNQPIVTRIGSPFLNAPVGGNLPAGATIVYDEVCIQAGHIWIGYNAYNGNRVYCPVRTCQGVPPNQQPGVAWGVFK

BlastP to GMEnzy

Production

1. Expressed by pBAD-TOPO in E. coli TOP15, Purified by cation-exchange chromatography

Activity

No activity data found on GM0186

Reference

1. Jun SY, Jung GM, Son JS, Yoon SJ, Choi YJ. (2011) Comparison of the antibacterial properties of phage endolysins SAL-1 and LysK. Antimicrob Agents Chemother. REF0048:1764-1767. [doi:10.1128/AAC.01097-10] [PMID:21263051] [FULL TEXT]

Comments

No comments found on GM0186

GMEnzy : A Genetically Modified Enzybiotic Database