Record in detail

General Info

lamp_id:L01A002969
Name:DEF13_ARATH
FullName:Defensin-like protein 13
Source:Arabidopsis thaliana
Mass:5667.5 Da
Sequence Length:51 aa
Isoelectric Point:8.41
Activity:Antifungal
Sequence
QKLCERPSGTWSGVCGNSNACKNQCINLEKARHGSCNYVFPAHKCICYFPC
Function:Confers broad-spectrum resistance to pathogens. Possesses antifungal activity sensitive to inorganic cations in vitro.

Cross-Linking

Cross-linking
1 Database:CAMP CAMPSQ912
2 Database:dbAMP dbAMP_10145
3 Database:DRAMP DRAMP00469
4 Database:Uniprot P30224
5 Database:PHY PHYT00004

Top similar AMPs

Top similar AMPs on LAMP
1. L03A000096 From 30 To 80 E-value: 3e-27 Score: 112
QKLCERPSGTWSGVCGNSNACKNQCINLEKARHGSCNYVFPAHKCICYFPC
2. L01A002125 From 30 To 80 E-value: 8e-27 Score: 110
QKLCERPSGTWSGVCGNNNACKNQCINLEKARHGSCNYVFPAHKCICYFPC
3. L12A06263| From 30 To 80 E-value: 1e-26 Score: 110
QKLCERPSGTWSGVCGNNNACKNQCINLEKARHGSCNYVFPAHKCICYFPC
4. L01A002969 From 1 To 51 E-value: 6e-26 Score: 107
QKLCERPSGTWSGVCGNSNACKNQCINLEKARHGSCNYVFPAHKCICYFPC
5. L05ADEF279 From 30 To 80 E-value: 1e-25 Score: 106
QKLCEKPSGTWSGVCGNSNACKNQCINLEGAKHGSCNYVFPAHKCICYFPC

Structure

Domains
1 Name:Gamma-thionin Interpro Link:IPR008176
2 Name:Knot1 Interpro Link:IPR003614

Structures

Activity

Antibacterial Activities
1 Target: Alternaria brassicola IC50: 10 μg/ml (1.76445 μM)
2 Target: Botrytis cinerea IC50: 3.9 μg/ml (0.688134 μM)
3 Target: Fusarium culmorum IC50: 3 μg/ml (0.529334 μM)
4 Target: Fusarium oxysporum f.sp.lycopersici IC50: 3 μg/ml (0.529334 μM)
5 Target: Pyricularia oryzae IC50: 0.25 μg/ml (0.0441112 μM)
6 Target: Verticiliium dahliae IC50: 1.5 μg/ml (0.264667 μM)

Toxicity

Toxicity

Reference

Reference
[1] Vanderleyden J.,Osborn R.W.,van Leuven F.,Torrekens S.,Terras F.R.G.,
Title:A new family of basic cysteine-rich plant antifungal proteins from Brassicaceae species.
Journal:FEBS Lett., 1993, 316, 233-240 [MEDLINE:93138130]
[2] Delseny M.,Grellet F.,Laudie M.,Raynal M.,Cooke R.,
Title:Further progress towards a catalogue of all Arabidopsis genes: analysis of a set of 5000 non-redundant ESTs.
Journal:Plant J., 1996, 9, 101-124 [MEDLINE:96158348]
[3] De Samblanx G.W.,Thomma B.P.H.J.,Terras F.R.G.,Eggermont K.,Penninckx I.A.M.A.,
Title:Pathogen-induced systemic activation of a plant defensin gene in Arabidopsis follows a salicylic acid-independent pathway.
Journal:Plant Cell, 1996, 8, 2309-2323 [PubMed:8989885]
[4] Meyerowitz E.M.,Clark S.E.,Williams R.W.,
Title:Genetic and physical characterization of a region of Arabidopsis chromosome 1 containing the CLAVATA1 gene.
Journal:Plant Mol. Biol., 1999, 39, 171-176 [MEDLINE:99178804]
[5] Kaul S.,Federspiel N.A.,Palm C.J.,Ecker J.R.,Theologis A.,
Title:Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.
Journal:Nature, 2000, 408, 816-820 [MEDLINE:21016719]
[6] Cock J.M.,Dumas C.,Miege C.,Vanoosthuyse V.,
Title:Two large Arabidopsis thaliana gene families are homologous to the Brassica gene superfamily that encodes pollen coat proteins and the male component of the self-incompatibility response.
Journal:Plant Mol. Biol., 2001, 46, 17-34 [MEDLINE:21330246]
[7] Thevissen K.,Cammue B.P.,Thomma B.P.H.J.,
Title:Plant defensins.
Journal:Planta, 2002, 216, 193-202 [PubMed:12447532]
[8] Shinn P.,Chen H.,Dale J.M.,Lim J.,Yamada K.,
Title:Empirical analysis of transcriptional activity in the Arabidopsis genome.
Journal:Science, 2003, 302, 842-846 [MEDLINE:22954850]
[9] VandenBosch K.A.,Paape T.D.,Graham M.A.,Silverstein K.A.T.,
Title:Genome organization of more than 300 defensin-like genes in Arabidopsis.
Journal:Plant Physiol., 2005, 138, 600-610 [PubMed:15955924]
[10] Cammue B.P.A.,Proost P.,Aerts A.M.,Delaure S.L.,Sels J.,
Title:Use of a PTGS-MAR expression system for efficient in planta production of bioactive Arabidopsis thaliana plant defensins.
Journal:Transgenic Res., 2007, 16, 531-538 [PubMed:17180735]

Comments

Comments