Record in detail


General Info

  • lamp_id:L01A000223
  • Name:KAB1_OLDAF
  • FullName:Kalata-B1
  • Source:Oldenlandia affinis
  • Mass:2916.3 Da
  • Sequence Length:29 aa
  • Isoelectric Point:5.93
  • Activity:Antibacterial, Antifungal
  • Sequence
        VCGETCVGGTCNTPGCTCSWPVCTRNGLP
  • Function:Probably participates in a plant defense mechanism. Has antibiotic activity. Has a diuretic effect. Has a uterotonic effect in humans. Active against the Gram-positive S. aureus with a minimum inhibition concentration of approximately 0.2 microM. Relatively ineffective against Gram-negative bacteria such as E.coli and P.aeruginosa. Inhibitory effect on the growth and development of larvae from H.punctigera. The unmodified form has hemolytic activity, the oxidized form lacks hemolytic activity. If the protein is linearized, hemolytic activity is lost.

Cross-Linking

Top similar AMPs

  • Top similar AMPs on LAMP
  • 1. L01A000223    From 1 To 29 E-value: 0.00000000004 Score: 58.5
        VCGETCVGGTCNTPGCTCSWPVCTRNGLP
  • 2. L02A001048    From 1 To 29 E-value: 0.00000000009 Score: 57.4
        ICGETCVGGTCNTPGCSCSWPVCTRNGLP
  • 3. L13A024911    From 1 To 29 E-value: 0.00000000009 Score: 57.4
        VCGETCVGGTCNTPGCTCSWPVCTRDGLP
  • 4. L02A000729    From 1 To 28 E-value: 0.0000000001 Score: 57
        CGETCVGGTCNTPGCTCSWPVCTRNGLP
  • 5. L02A001806    From 1 To 28 E-value: 0.0000000003 Score: 55.8
        CGETCVGGTCNTPGCACSWPVCTRNGLP

Structure

  •   Domains
  •   1  Name:Cyclotide    Interpro Link:IPR005535
  •   2  Name:Cyclotide_moebius_CS    Interpro Link:IPR012324
  •   Structures

Activity

  •   Antibacterial Activities
  •   1  Target:  K. oxytoca  MIC:  159.81 μg/ml  (54.7989 μM)  
  •   2  Target:  S. aureus  MIC:  0.76 μg/ml  (0.260604 μM)  
  •   3  Target:  M. luteus  MIC:  117.82 μg/ml  (40.4005 μM)  
  •   4  Target:  C. kefyr  MIC:  62.41 μg/ml  (21.4004 μM)  

Toxicity

  •   Toxicity

    •     No toxicity records found on LAMP database

Reference

  •   Reference
  •   [1]  Juul J.,Sletten K.,Campbell I.D.,Craik D.J.,Saether O.,
  •   Title:Elucidation of the primary and three-dimensional structure of the uterotonic polypeptide kalata B1.
  •   Journal:Biochemistry, 1995, 34, 4147-4158  [MEDLINE:95217878]
  •   [2]  Chiu K.-W.,Yang J.-L.,Lu Y.-A.,Tam J.P.,
  •   Title:An unusual structural motif of antimicrobial peptides containing end-to-end macrocycle and cystine-knot disulfides.
  •   Journal:Proc. Natl. Acad. Sci. U.S.A., 1999, 96, 8913-8918  [MEDLINE:99362685]
  •   [3]  Anderson M.A.,Craik D.J.,Waine C.,West J.,Jennings C.V.,
  •   Title:Biosynthesis and insecticidal properties of plant cyclotides: the cyclic knotted proteins from Oldenlandia affinis.
  •   Journal:Proc. Natl. Acad. Sci. U.S.A., 2001, 98, 10614-10619  [MEDLINE:21438013]
  •   [4]  Volkman B.F.,Sletten K.,Gran L.,Skjeldal L.,
  •   Title:Refined structure and metal binding site of the kalata B1 peptide.
  •   Journal:Arch. Biochem. Biophys., 2002, 399, 142-148  [PubMed:11888199]
  •   [5]  Craik D.J.,Clark R.J.,Daly N.L.,
  •   Title:Disulfide folding pathways of cystine knot proteins. Tying the knot within the circular backbone of the cyclotides.
  •   Journal:J. Biol. Chem., 2003, 278, 6314-6322  [PubMed:12482862]
  •   [6]  Craik D.J.,Sando L.,Clark R.J.,Daly N.L.,Barry D.G.,
  •   Title:Linearization of a naturally occurring circular protein maintains structure but eliminates hemolytic activity.
  •   Journal:Biochemistry, 2003, 42, 6688-6695  [PubMed:12779323]
  •   [7]  Craik D.J.,Waine C.,Plan M.R.R.,Daly N.L.,Rosengren K.J.,
  •   Title:Twists, knots, and rings in proteins. Structural definition of the cyclotide framework.
  •   Journal:J. Biol. Chem., 2003, 278, 8606-8616  [PubMed:12482868]
  •   [8]  Colgrave M.L.,Daly N.L.,Clark R.J.,Goeransson U.,Plan M.R.R.,
  •   Title:The cyclotide fingerprint in Oldenlandia affinis: elucidation of chemically modified, linear and novel macrocyclic peptides.
  •   Journal:ChemBioChem, 2007, 8, 1001-1011  [PubMed:17534989]

Comments

  •   Comments

    •     No comments found on LAMP database



CAPTCHA Image   Reload Image
Enter Code*: