Record in detail


General Info

  • lamp_id:L01A002961
  • Name:SAPE_SARPE
  • FullName:Sapecin
  • Source:Sarcophaga peregrina
  • Mass:4080.6 Da
  • Sequence Length:40 aa
  • Isoelectric Point:8.39
  • Activity:Antibacterial
  • Sequence
        ATCDLLSGTGINHSACAAHCLLRGNRGGYCNGKAVCVCRN
  • Function:Sapecins, which are potent bactericidal proteins, are produced in response to injury. Sapecin is cytotoxic to Gram-positive bacteria, and to a lesser extent against Gram-negative bacteria.

Cross-Linking

Top similar AMPs

  • Top similar AMPs on LAMP
  • 1. L03A000167    From 55 To 94 E-value: 9e-19 Score: 84
        ATCDLLSGTGINHSACAAHCLLRGNRGGYCNGKAVCVCRN
  • 2. L03A000131    From 55 To 94 E-value: 4e-18 Score: 81.6
        ATCDLLSGTGINHSACAAHCLLRGNRGGYCNGKGVCVCRN
  • 3. L01A002961    From 1 To 40 E-value: 1e-17 Score: 80.1
        ATCDLLSGTGINHSACAAHCLLRGNRGGYCNGKAVCVCRN
  • 4. L02A000216    From 1 To 40 E-value: 4e-17 Score: 78.6
        ATCDLLSGTGINHSACAAHCLLRGNRGGYCNGKGVCVCRN
  • 5. L13A015013    From 1 To 40 E-value: 1e-16 Score: 77
        ATCDLLSGTGIKHSACAAHCLLRGNRGGYCNGRAICVCRN

Structure

  •   Domains
  •   1  Name:Defensin_insect    Interpro Link:IPR017982
  •   2  Name:Defensin_invertebrate/fungal    Interpro Link:IPR001542
  •   3  Name:Knot1    Interpro Link:IPR003614
  •   Structures

Activity

  •   Antibacterial Activities
  •   1  Target:  Staphylococcus aureus FDA209P  MIC:  5 μg/ml  (1.22531 μM)  
  •   2  Target:  Staphylococcus smith  MIC:  5 μg/ml  (1.22531 μM)  
  •   3  Target:  Micrococcus luteus FDA16  MIC:  1.2 μg/ml  (0.294074 μM)  
  •   4  Target:  Micrococcus luteus IF03333  MIC:  1.2 μg/ml  (0.294074 μM)  
  •   5  Target:  Micrococcus luteus PC11001  MIC:  10 μg/ml  (2.45062 μM)  
  •   6  Target:  Bacillus subtilis NRRI B-558  MIC:  39 μg/ml  (9.55742 μM)  
  •   7  Target:  Bacillus subtilis PC1219  MIC:  20 μg/ml  (4.90124 μM)  
  •   8  Target:  Mycobacterium smegmatis ATCC607  MIC:  78 μg/ml  (19.1148 μM)  
  •   9  Target:  Corynebacterium bouis 1810  MIC:  2.5 μg/ml  (0.612655 μM)  
  •   10  Target:  Corynebacterium michiganense  MIC:  2.5 μg/ml  (0.612655 μM)  
  •   11  Target:  Xantbmonas oryzae  MIC:  20 μg/ml  (4.90124 μM)  
  •   12  Target:  Pseudomonas lachrymans  MIC:  78 μg/ml  (19.1148 μM)  
  •   13  Target:  Escherichia coli NIHJ  MIC:  78 μg/ml  (19.1148 μM)  
  •   14  Target:  Shigella sonnei JS11746  MIC:  78 μg/ml  (19.1148 μM)  
  •   15  Target:  Proteus vulgaris O X19  MIC:  78 μg/ml  (19.1148 μM)  

Toxicity

  •   Toxicity

    •     No toxicity records found on LAMP database

Reference

  •   Reference
  •   [1]  Natori S.,Matsuyama K.,
  •   Title:Molecular cloning of cDNA for sapecin and unique expression of the sapecin gene during the development of Sarcophaga peregrina.
  •   Journal:J. Biol. Chem., 1988, 263, 17117-17121  [MEDLINE:89034216]
  •   [2]  Natori S.,Matsuyama K.,
  •   Title:Purification of three antibacterial proteins from the culture medium of NIH-Sape-4, an embryonic cell line of Sarcophaga peregrina.
  •   Journal:J. Biol. Chem., 1988, 263, 17112-17116  [MEDLINE:89034215]
  •   [3]  Kohda D.,Komano H.,Kuzuhara T.,Shimada I.,Hanzawa H.,
  •   Title:1H nuclear magnetic resonance study of the solution conformation of an antibacterial protein, sapecin.
  •   Journal:FEBS Lett., 1990, 269, 413-420  [MEDLINE:90382590]
  •   [4]  Natori S.,Matsuyama K.,Nakajima Y.,Kuzuhara T.,
  •   Title:Determination of the disulfide array in sapecin, an antibacterial peptide of Sarcophaga peregrina (flesh fly).
  •   Journal:J. Biochem., 1990, 107, 514-518  [MEDLINE:90292974]

Comments

  •   Comments

    •     No comments found on LAMP database



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